Multiple high-throughput analyses monitor the response of E-coli to perturbations

被引：508

作者：

Ishii, Nobuyoshi

Nakahigashi, Kenji

Baba, Tomoya

Robert, Martin

Soga, Tomoyoshi

Kanai, Akio

Hirasawa, Takashi

Naba, Miki

Hirai, Kenta

Hoque, Aminul

Ho, Pei Yee

Kakazu, Yuji

Sugawara, Kaori

Igarashi, Saori

Harada, Satoshi

Masuda, Takeshi

Sugiyama, Naoyuki

Togashi, Takashi

Hasegawa, Miki

Takai, Yuki

Yugi, Katsuyuki

Arakawa, Kazuharu

Iwata, Nayuta

Toya, Yoshihiro

Nakayama, Yoichi

Nishioka, Takaaki

Shimizu, Kazuyuki

Mori, Hirotada

Tomita, Masaru ^{[1
]}

机构：

[1] Keio Univ, Inst Adv Biosci, Yamagata 9970017, Japan

[2] Keio Univ, Grad Sch Media & Governance, Syst Biol Program, Fujisawa, Kanagawa 2528520, Japan

[3] Nara Inst Sci & Technol, Grad Sch Biol Sci, Nara 6300101, Japan

[4] Kyoto Univ, Grad Sch Agr, Kyoto 6068502, Japan

[5] Kyushu Inst Technol, Dept Biosci & Bioinformat, Fukuoka 8208502, Japan

[6] Human Metabolome Technol Inc, Tsuruoka, Japan

来源：

SCIENCE | 2007年 / 316卷 / 5824期

关键词：

D O I：

10.1126/science.1132067

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of Escherichia coli cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, E. coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations.

引用

页码：593 / 597

页数：5

共 19 条

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