Transforming growth factor-β1 stimulates protein kinase A in mesangial cells

被引：64

作者：

Wang, LW ^{[1
]}

Zhu, YQ ^{[1
]}

Sharma, K ^{[1
]}

机构：

[1] Thomas Jefferson Univ, Sch Med, Dept Med, Div Nephrol, Philadelphia, PA 19107 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1998年 / 273卷 / 14期

关键词：

D O I：

10.1074/jbc.273.14.8522

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We recently demonstrated that transforming growth factor-beta (TGF-beta) stimulates phosphorylation of the type I inositol 1,4,5-trisphosphate receptor (Sharma, K., Wang, L., Zhu, Y., Bokkala, S., and Joseph, S. (1997) J. Biol. Chem. 272, 14617-14623), possibly via protein kinase A (PKA) activation in murine mesangial cells. In the present study, we evaluated whether TGF-beta stimulates PKA activation. Utilizing a specific PKA kinase assay, me found that TGF-beta increases PRA activity by 3-fold within 15 min of TGF-beta treatment, and the enhanced kinase activity was completely reversed by the inhibitory peptide for PKA (PKI; 1 mu M). In mesangial cells transfected with a PKI expression vector, enhanced PKA activity could not be demonstrated with TGF-beta 1 treatment, TGF-beta 1 was also found to stimulate translocation of the alpha-catalytic subunit of PKA to the nucleus by Western analysis of nuclear protein as well as by confocal microscopy. TGF-beta 1-mediated phosphorylation of cAMP response element-binding protein was completely reversed by H-89 (3 mu M), a specific inhibitor of PKA. Stimulation of fibronectin mRNA by TGF-beta 1 was also attenuated in cells overexpressing PKI. We thus conclude that TGF-beta stimulates the PKA signaling pathway in mesangial cells and that PKA activation contributes to TGF-beta stimulation of cAMP response element-binding protein phosphorylation and fibronectin expression.

引用

页码：8522 / 8527

页数：6

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