An interaction between two RNA binding proteins, Nab2 and Pub1, links mRNA Processing/Export and mRNA stability

被引:18
作者
Apponi, Luciano H.
Kelly, Seth M.
Harreman, Michelle T.
Lehner, Alexander N.
Corbett, Anita H.
Valentini, Sandro R.
机构
[1] Sao Paulo State Univ, UNESP, Sch Pharmaceut Sci, Dept Biol Sci, BR-14801902 Araraquara, SP, Brazil
[2] Emory Univ, Sch Med, Dept Biochem, Atlanta, GA 30322 USA
关键词
D O I
10.1128/MCB.00881-07
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
mRNA stability is modulated by elements in the mRNA transcript and their cognate RNA binding proteins. Poly(U) binding protein 1 (Pub1) is a cytoplasmic Saccharomyces cerevisiae mRNA binding protein that stabilizes transcripts containing AU-rich elements (AREs) or stabilizer elements (STEs). In a yeast two-hybrid screen, we identified nuclear poly(A) binding protein 2 (Nab2) as being a Pub1-interacting protein. Nab2 is an essential nucleocytoplasmic shuttling mRNA binding protein that regulates poly(A) tail length and mRNA export. The interaction between Pub1 and Nab2 was confirmed by copurification and in vitro binding assays. The interaction is mediated by the Nab2 zinc finger domain. Analysis of the functional link between these proteins reveals that Nab2, like Pub1, can modulate the stability of specific mRNA transcripts. The half-life of the RPS16B transcript, an ARE-like sequence-containing Pub1 target, is decreased in both nab2-1 and nab2-67 mutants. In contrast, GCN4, an STE-containing Pub1 target, is not affected. Similar results were obtained for other ARE- and STE-containing Pub1 target transcripts. Further analysis reveals that the ARE-like sequence is necessary for Nab2-mediated transcript stabilization. These results suggest that Nab2 functions together with Pub1 to modulate mRNA stability and strengthen a model where nuclear events are coupled to the control of mRNA turnover in the cytoplasm.
引用
收藏
页码:6569 / 6579
页数:11
相关论文
共 64 条
[11]   Eukaryotic mRNA decapping [J].
Coller, J ;
Parker, R .
ANNUAL REVIEW OF BIOCHEMISTRY, 2004, 73 :861-890
[12]   Nonsense-mediated mRNA decay: molecular insights and mechanistic variations across species [J].
Conti, E ;
Izaurralde, E .
CURRENT OPINION IN CELL BIOLOGY, 2005, 17 (03) :316-325
[13]   EXTREME INSTABILITY OF MYC MESSENGER-RNA IN NORMAL AND TRANSFORMED HUMAN-CELLS [J].
DANI, C ;
BLANCHARD, JM ;
PIECHACZYK, M ;
ELSABOUTY, S ;
MARTY, L ;
JEANTEUR, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (22) :7046-7050
[14]   Messenger-RNA-binding proteins and the messages they carry [J].
Dreyfuss, G ;
Kim, VN ;
Kataoka, N .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2002, 3 (03) :195-205
[15]   Yeast poly(A)-binding protein, Pab1, and PAN, A poly(A) nuclease complex recruited by Pab1, connect mRNA biogenesis to export [J].
Dunn, EF ;
Hammell, CM ;
Hodge, CA ;
Cole, CN .
GENES & DEVELOPMENT, 2005, 19 (01) :90-103
[16]   Global analysis of Pup1p targets reveals a coordinate control of gene expression through modulation of binding and stability [J].
Duttagupta, R ;
Tian, B ;
Wilusz, CJ ;
Khounh, DT ;
Soteropoulos, P ;
Ouyang, M ;
Dougherty, JP ;
Peltz, SW .
MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (13) :5499-5513
[17]   Overexpression of HuR, a nuclear-cytoplasmic shuttling protein, increases the in vivo stability of ARE-containing mRNAs [J].
Fan, XHC ;
Steitz, JA .
EMBO JOURNAL, 1998, 17 (12) :3448-3460
[18]   A nuclear translation-like factor elF4AIII is recruited to the mRNA during splicing and functions in nonsense-mediated decay [J].
Ferraiuolo, MA ;
Lee, CS ;
Ler, LW ;
Hsu, JL ;
Costa-Mattioli, M ;
Luo, MJ ;
Reed, R ;
Sonenberg, N .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (12) :4118-4123
[19]   The highways and byways of mRNA decay [J].
Garneau, Nicole L. ;
Wilusz, Jeffrey ;
Wilusz, Carol J. .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2007, 8 (02) :113-126
[20]   Nonsense-mediated mRNA decay in Saccharomyces cerevisiae [J].
González, CI ;
Bhattacharya, A ;
Wang, WR ;
Peltz, SW .
GENE, 2001, 274 (1-2) :15-25