Cytokine profiles of T-lymphocytes from gingival tissues with pathological pocketing

被引:120
作者
Takeichi, O
Haber, J
Kawai, T
Smith, DJ
Moro, I
Taubman, MA
机构
[1] Forsyth Inst, Dept Immunol, Boston, MA 02115 USA
[2] Nihon Univ, Sch Dent, Dept Endodont, Tokyo 101, Japan
[3] Nihon Univ, Sch Dent, Dept Pathol, Tokyo 101, Japan
关键词
cytokine; periodontal disease; gingival tissue; T-lymphocytes; Th1; Th2; CD4(+); CD8(+);
D O I
10.1177/00220345000790080401
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Periodontal disease is an infection in which destruction occurs at sites remote from the infection, resulting in pathological pocketing. Intervening between the infection and the destruction is a dense mononuclear inflammatory infiltrate. It has been suggested that this infiltrate might have characteristics and the destructive potential of Th1-type T lymphocytes. To ascertain the nature of the infiltrates we investigated the expression of mRNA for IL-2, IL-5, and IFN-gamma by gingival mononuclear cells (GMC) from healthy (n = 8) or adult periodontitis (AP) patients (n = 25) by using cytokine-specific reverse-transcription/polymerase-L chain-reaction (RT-PCR). GMC, as obtained from patients' tissues, expressed IL-2, IFN-gamma, or IL-5 mRNA. Significantly higher proportions of GMC from AP patients expressed IL-2 and IFN-gamma mRNA than did those from healthy subjects. IFN-gamma was the most consistent cytokine message detected. In other experiments, gingival T-lymphocytes (n = 12) and CD4(+) and CD8(+) gingival T-lymphocytes (n = 16) were isolated from gingival tissues removed surgically from AP patients. AP gingival T-lymphocytes expressed mRNA for IL-2, IFN-gamma, or IL-6 prior to stimulation. After stimulation with Con A, the cells significantly up-regulated IL-5 and IL-6 message expression. Both CD4(+) and CD8(+) gingival T-lymphocytes expressed IFN-gamma, IL-5, and some IL-2. This cumulative cytokine profile observed in these experiments is consistent with the predominance of Th1-type cells in pathological tissues and with Th2-type cells, which can also be present, being up-regulated under appropriate stimulation. Importantly, CD4(+) and CD8(+) lymphocytes were shown to express T1- and T2-type cytokine message, emphasizing the potential for CD8(+) T-lymphocytes to participate in periodontal disease pathology.
引用
收藏
页码:1548 / 1555
页数:8
相关论文
共 44 条
[1]   Functional diversity of helper T lymphocytes [J].
Abbas, AK ;
Murphy, KM ;
Sher, A .
NATURE, 1996, 383 (6603) :787-793
[2]  
Assuma R, 1998, J IMMUNOL, V160, P403
[3]  
CARTER LL, 1995, J IMMUNOL, V155, P1028
[4]   Type 1 and Type 2: A fundamental dichotomy for all T-cell subsets [J].
Carter, LL ;
Dutton, RW .
CURRENT OPINION IN IMMUNOLOGY, 1996, 8 (03) :336-342
[5]  
CHER DJ, 1987, J IMMUNOL, V138, P3688
[6]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[7]   PREVENTION OF PRE-PCR MIS-PRIMING AND PRIMER DIMERIZATION IMPROVES LOW-COPY-NUMBER AMPLIFICATIONS [J].
CHOU, Q ;
RUSSELL, M ;
BIRCH, DE ;
RAYMOND, J ;
BLOCH, W .
NUCLEIC ACIDS RESEARCH, 1992, 20 (07) :1717-1723
[8]   PHENOTYPIC AND FUNCTIONAL-ANALYSIS OF T-CELLS EXTRACTED FROM CHRONICALLY INFLAMED HUMAN PERIODONTAL TISSUES [J].
COLE, KL ;
SEYMOUR, GJ ;
POWELL, RN .
JOURNAL OF PERIODONTOLOGY, 1987, 58 (08) :569-573
[9]   MAXIMIZING SENSITIVITY AND SPECIFICITY OF PCR BY PREAMPLIFICATION HEATING [J].
DAQUILA, RT ;
BECHTEL, LJ ;
VIDELER, JA ;
ERON, JJ ;
GORCZYCA, P ;
KAPLAN, JC .
NUCLEIC ACIDS RESEARCH, 1991, 19 (13) :3749-3749
[10]   PURIFIED PROTEIN DERIVATIVE OF MYCOBACTERIUM-TUBERCULOSIS AND EXCRETORY-SECRETORY ANTIGEN(S) OF TOXOCARA-CANIS EXPAND INVITRO HUMAN T-CELLS WITH STABLE AND OPPOSITE (TYPE-1 T-HELPER OR TYPE-2 T-HELPER) PROFILE OF CYTOKINE PRODUCTION [J].
DELPRETE, GF ;
DECARLI, M ;
MASTROMAURO, C ;
BIAGIOTTI, R ;
MACCHIA, D ;
FALAGIANI, P ;
RICCI, M ;
ROMAGNANI, S .
JOURNAL OF CLINICAL INVESTIGATION, 1991, 88 (01) :346-350