Cytokine profiles of T-lymphocytes from gingival tissues with pathological pocketing

被引:120
作者
Takeichi, O
Haber, J
Kawai, T
Smith, DJ
Moro, I
Taubman, MA
机构
[1] Forsyth Inst, Dept Immunol, Boston, MA 02115 USA
[2] Nihon Univ, Sch Dent, Dept Endodont, Tokyo 101, Japan
[3] Nihon Univ, Sch Dent, Dept Pathol, Tokyo 101, Japan
关键词
cytokine; periodontal disease; gingival tissue; T-lymphocytes; Th1; Th2; CD4(+); CD8(+);
D O I
10.1177/00220345000790080401
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Periodontal disease is an infection in which destruction occurs at sites remote from the infection, resulting in pathological pocketing. Intervening between the infection and the destruction is a dense mononuclear inflammatory infiltrate. It has been suggested that this infiltrate might have characteristics and the destructive potential of Th1-type T lymphocytes. To ascertain the nature of the infiltrates we investigated the expression of mRNA for IL-2, IL-5, and IFN-gamma by gingival mononuclear cells (GMC) from healthy (n = 8) or adult periodontitis (AP) patients (n = 25) by using cytokine-specific reverse-transcription/polymerase-L chain-reaction (RT-PCR). GMC, as obtained from patients' tissues, expressed IL-2, IFN-gamma, or IL-5 mRNA. Significantly higher proportions of GMC from AP patients expressed IL-2 and IFN-gamma mRNA than did those from healthy subjects. IFN-gamma was the most consistent cytokine message detected. In other experiments, gingival T-lymphocytes (n = 12) and CD4(+) and CD8(+) gingival T-lymphocytes (n = 16) were isolated from gingival tissues removed surgically from AP patients. AP gingival T-lymphocytes expressed mRNA for IL-2, IFN-gamma, or IL-6 prior to stimulation. After stimulation with Con A, the cells significantly up-regulated IL-5 and IL-6 message expression. Both CD4(+) and CD8(+) gingival T-lymphocytes expressed IFN-gamma, IL-5, and some IL-2. This cumulative cytokine profile observed in these experiments is consistent with the predominance of Th1-type cells in pathological tissues and with Th2-type cells, which can also be present, being up-regulated under appropriate stimulation. Importantly, CD4(+) and CD8(+) lymphocytes were shown to express T1- and T2-type cytokine message, emphasizing the potential for CD8(+) T-lymphocytes to participate in periodontal disease pathology.
引用
收藏
页码:1548 / 1555
页数:8
相关论文
共 44 条
[31]   INTERLEUKIN-2 PRODUCTION AND BONE-RESORPTION ACTIVITY INVITRO BY UNSTIMULATED LYMPHOCYTES EXTRACTED FROM CHRONICALLY-INFLAMED HUMAN PERIODONTAL TISSUES [J].
SEYMOUR, GJ ;
COLE, KL ;
POWELL, RN ;
LEWINS, E ;
CRIPPS, AW ;
CLANCY, RL .
ARCHIVES OF ORAL BIOLOGY, 1985, 30 (06) :481-484
[32]  
Southern E, 1979, Methods Enzymol, V68, P152
[33]   DETECTION OF SPECIFIC SEQUENCES AMONG DNA FRAGMENTS SEPARATED BY GEL-ELECTROPHORESIS [J].
SOUTHERN, EM .
JOURNAL OF MOLECULAR BIOLOGY, 1975, 98 (03) :503-+
[34]   PHENOTYPIC ANALYSES OF MONONUCLEAR-CELLS RECOVERED FROM HEALTHY AND DISEASED HUMAN PERIODONTAL TISSUES [J].
STOUFI, ED ;
TAUBMAN, MA ;
EBERSOLE, JL ;
SMITH, DJ ;
STASHENKO, PP .
JOURNAL OF CLINICAL IMMUNOLOGY, 1987, 7 (03) :235-245
[35]   PREPARATION AND CHARACTERIZATION OF HUMAN GINGIVAL CELLS [J].
STOUFI, ED ;
TAUBMAN, MA ;
EBERSOLE, JL ;
SMITH, DJ .
JOURNAL OF PERIODONTAL RESEARCH, 1987, 22 (02) :144-149
[36]   HUMAN POLYMORPHONUCLEAR LEUKOCYTES DERIVED FROM CHRONICALLY INFLAMED TISSUE EXPRESS INFLAMMATORY CYTOKINES IN-VIVO [J].
TAKEICHI, O ;
SAITO, I ;
TSURUMACHI, T ;
SAITO, T ;
MORO, I .
CELLULAR IMMUNOLOGY, 1994, 156 (02) :296-309
[37]  
TAUBMAN MA, 1994, MOLECULAR PATHOGENESIS OF PERIODONTAL DISEASE, P147
[38]  
TSICOPOULOS A, 1992, J IMMUNOL, V148, P2058
[39]   THE REGULATION OF INTERLEUKIN-5 AND INTERLEUKIN-3 GENE-EXPRESSION IN HUMAN T-CELLS [J].
VANSTRAATEN, JFM ;
DOKTER, WHA ;
STULP, BK ;
VELLENGA, E .
CYTOKINE, 1994, 6 (03) :229-234
[40]   QUANTITATION OF MESSENGER-RNA BY THE POLYMERASE CHAIN-REACTION [J].
WANG, AM ;
DOYLE, MV ;
MARK, DF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (24) :9717-9721