Cytokine profiles of T-lymphocytes from gingival tissues with pathological pocketing

被引:120
作者
Takeichi, O
Haber, J
Kawai, T
Smith, DJ
Moro, I
Taubman, MA
机构
[1] Forsyth Inst, Dept Immunol, Boston, MA 02115 USA
[2] Nihon Univ, Sch Dent, Dept Endodont, Tokyo 101, Japan
[3] Nihon Univ, Sch Dent, Dept Pathol, Tokyo 101, Japan
关键词
cytokine; periodontal disease; gingival tissue; T-lymphocytes; Th1; Th2; CD4(+); CD8(+);
D O I
10.1177/00220345000790080401
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Periodontal disease is an infection in which destruction occurs at sites remote from the infection, resulting in pathological pocketing. Intervening between the infection and the destruction is a dense mononuclear inflammatory infiltrate. It has been suggested that this infiltrate might have characteristics and the destructive potential of Th1-type T lymphocytes. To ascertain the nature of the infiltrates we investigated the expression of mRNA for IL-2, IL-5, and IFN-gamma by gingival mononuclear cells (GMC) from healthy (n = 8) or adult periodontitis (AP) patients (n = 25) by using cytokine-specific reverse-transcription/polymerase-L chain-reaction (RT-PCR). GMC, as obtained from patients' tissues, expressed IL-2, IFN-gamma, or IL-5 mRNA. Significantly higher proportions of GMC from AP patients expressed IL-2 and IFN-gamma mRNA than did those from healthy subjects. IFN-gamma was the most consistent cytokine message detected. In other experiments, gingival T-lymphocytes (n = 12) and CD4(+) and CD8(+) gingival T-lymphocytes (n = 16) were isolated from gingival tissues removed surgically from AP patients. AP gingival T-lymphocytes expressed mRNA for IL-2, IFN-gamma, or IL-6 prior to stimulation. After stimulation with Con A, the cells significantly up-regulated IL-5 and IL-6 message expression. Both CD4(+) and CD8(+) gingival T-lymphocytes expressed IFN-gamma, IL-5, and some IL-2. This cumulative cytokine profile observed in these experiments is consistent with the predominance of Th1-type cells in pathological tissues and with Th2-type cells, which can also be present, being up-regulated under appropriate stimulation. Importantly, CD4(+) and CD8(+) lymphocytes were shown to express T1- and T2-type cytokine message, emphasizing the potential for CD8(+) T-lymphocytes to participate in periodontal disease pathology.
引用
收藏
页码:1548 / 1555
页数:8
相关论文
共 44 条
[21]  
LUNDQVIST C, 1994, J IMMUNOL, V153, P2302
[22]  
Lundqvist Carina A., 1992, Regional Immunology, V4, P255
[23]  
McGhee M L, 1989, Reg Immunol, V2, P103
[24]  
MOSMANN TR, 1989, ANNU REV IMMUNOL, V7, P145, DOI 10.1146/annurev.iy.07.040189.001045
[25]   IDENTIFICATION AND DISTRIBUTION OF IMMUNOCOMPETENT CELLS IN INFLAMED GINGIVA OF HUMAN CHRONIC PERIODONTITIS [J].
OKADA, H ;
KIDA, T ;
YAMAGAMI, H .
INFECTION AND IMMUNITY, 1983, 41 (01) :365-374
[26]   THE ROLE OF INFLAMMATORY MEDIATORS IN THE PATHOGENESIS OF PERIODONTAL-DISEASE [J].
PAGE, RC .
JOURNAL OF PERIODONTAL RESEARCH, 1991, 26 (03) :230-242
[27]  
PAGE RC, 1976, LAB INVEST, V34, P235
[28]  
PALIARD X, 1988, J IMMUNOL, V141, P849
[29]   HUMAN TH1 AND TH2 SUBSETS - DOUBT NO MORE [J].
ROMAGNANI, S .
IMMUNOLOGY TODAY, 1991, 12 (08) :256-257
[30]   ROLE OF CYTOKINES AND CD4+ T-CELL SUBSETS IN THE REGULATION OF PARASITE IMMUNITY AND DISEASE [J].
SCOTT, P ;
PEARCE, E ;
CHEEVER, AW ;
COFFMAN, RL ;
SHER, A .
IMMUNOLOGICAL REVIEWS, 1989, 112 :161-182