Spectrum of cytokine gene expression in intestinal mucosal lesions of Crohn's disease and ulcerative colitis

被引：51

作者：

Funakoshi, K ^{[1
]}

Sugimura, K ^{[1
]}

Anezaki, K ^{[1
]}

Bannai, H ^{[1
]}

Ishizuka, K ^{[1
]}

Asakura, H ^{[1
]}

机构：

[1] Niigata Univ, Sch Med, Dept Internal Med 3, Niigata 951, Japan

来源：

DIGESTION | 1998年 / 59卷 / 01期

关键词：

Crohn's disease; ulcerative colitis; cytokine mRNA; reverse transcription-polymerase chain reaction;

D O I：

10.1159/000007470

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background/Aims: We investigated the mRNA expression of spectrum of cytokines in the colonic mucosa in inflammatory bowel disease (IBD). Methods: The expression of cytokine gene was evaluated by using the reverse transcription-polymerase chain reaction and the radioactivity of amplified cDNA standardized by coamplified beta-actin cDNA. Results: Crohn's disease and ulcerative colitis showed significantly increased expression of IL-1 beta, IL-6, IL-8 and TNF-alpha mRNA as compared with controls (p < 0.05). Conclusion: Proinflammatory cytokines such as IL-1 beta, IL-6, IL-8 and TNF-alpha are closely involved in the immune abnormalities of inflammatory mucosal lesions in IBD.

引用

页码：73 / 78

页数：6

共 37 条

[1] IMMUNOHISTOLOGICAL STUDIES OF SURFACE-ANTIGEN ON COLONIC LYMPHOID-CELLS IN NORMAL AND INFLAMED MUCOSA - COMPARISON OF FOLLICULAR AND LAMINA PROPRIA LYMPHOCYTES
ALLISON, MC
POULTER, LW
DHILLON, AP
POUNDER, RE
[J]. GASTROENTEROLOGY, 1990, 99 (02) : 421 - 430
[2] TUMOR-NECROSIS-FACTOR-ALPHA IN STOOL AS A MARKER OF INTESTINAL INFLAMMATION
BRAEGGER, CP
NICHOLLS, S
MURCH, SH
STEPHENS, S
MACDONALD, TT
[J]. LANCET, 1992, 339 (8785) : 89 - 91
[3] BREESE E, 1993, IMMUNOLOGY, V78, P127
[4] TUMOR-NECROSIS-FACTOR ALPHA-PRODUCING CELLS IN THE INTESTINAL-MUCOSA OF CHILDREN WITH INFLAMMATORY BOWEL-DISEASE
BREESE, EJ
MICHIE, CA
NICHOLLS, SW
MURCH, SH
WILLIAMS, CB
DOMIZIO, P
WALKERSMITH, JA
MACDONALD, TT
[J]. GASTROENTEROLOGY, 1994, 106 (06) : 1455 - 1466
[5] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[6] DIFFERENTIAL EXPRESSION OF CD25 (INTERLEUKIN-2 RECEPTOR) ON LAMINA PROPRIA T-CELLS AND MACROPHAGES IN THE INTESTINAL LESIONS IN CROHNS-DISEASE AND ULCERATIVE-COLITIS
CHOY, MY
WALKERSMITH, JA
WILLIAMS, CB
MACDONALD, TT
[J]. GUT, 1990, 31 (12) : 1365 - 1370
[7] DEDOMBAL FT, 1993, INFLAMM BOWEL DIS, P127
[8] FIOCCHI C, 1984, GASTROENTEROLOGY, V86, P734
[9] FIORENTINO DF, 1991, J IMMUNOL, V146, P3444
[10] Fuss IJ, 1996, J IMMUNOL, V157, P1261

← 1 2 3 4 →