Spectrum of cytokine gene expression in intestinal mucosal lesions of Crohn's disease and ulcerative colitis

被引：51

作者：

Funakoshi, K ^{[1
]}

Sugimura, K ^{[1
]}

Anezaki, K ^{[1
]}

Bannai, H ^{[1
]}

Ishizuka, K ^{[1
]}

Asakura, H ^{[1
]}

机构：

[1] Niigata Univ, Sch Med, Dept Internal Med 3, Niigata 951, Japan

来源：

DIGESTION | 1998年 / 59卷 / 01期

关键词：

Crohn's disease; ulcerative colitis; cytokine mRNA; reverse transcription-polymerase chain reaction;

D O I：

10.1159/000007470

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background/Aims: We investigated the mRNA expression of spectrum of cytokines in the colonic mucosa in inflammatory bowel disease (IBD). Methods: The expression of cytokine gene was evaluated by using the reverse transcription-polymerase chain reaction and the radioactivity of amplified cDNA standardized by coamplified beta-actin cDNA. Results: Crohn's disease and ulcerative colitis showed significantly increased expression of IL-1 beta, IL-6, IL-8 and TNF-alpha mRNA as compared with controls (p < 0.05). Conclusion: Proinflammatory cytokines such as IL-1 beta, IL-6, IL-8 and TNF-alpha are closely involved in the immune abnormalities of inflammatory mucosal lesions in IBD.

引用

页码：73 / 78

页数：6

共 37 条

[11] CYTOKINE MESSENGER-RNA PROFILES IN INFLAMMATORY BOWEL-DISEASE MUCOSA DETECTED BY POLYMERASE CHAIN-REACTION AMPLIFICATION
ISAACS, KL
SARTOR, RB
HASKILL, S
[J]. GASTROENTEROLOGY, 1992, 103 (05) : 1587 - 1595
[12] KUCHARZIK T, 1995, CLIN EXP IMMUNOL, V100, P452
[13] KUSUGAMI K, 1989, GASTROENTEROLOGY, V97, P1
[14] ROLE OF INTERLEUKIN-1 IN INFLAMMATORY BOWEL-DISEASE - ENHANCED PRODUCTION DURING ACTIVE DISEASE
LIGUMSKY, M
SIMON, PL
KARMELI, F
RACHMILEWITZ, D
[J]. GUT, 1990, 31 (06) : 686 - 689
[15] ENHANCED PRODUCTION OF INTERLEUKIN-1-BETA BY MONONUCLEAR-CELLS ISOLATED FROM MUCOSA WITH ACTIVE ULCERATIVE-COLITIS OF CROHNS-DISEASE
MAHIDA, YR
WU, K
JEWELL, DP
[J]. GUT, 1989, 30 (06) : 835 - 838
[16] MALEFYT RD, 1991, J EXP MED, V174, P1209
[17] MATUURA T, 1993, GASTROENTEROLOGY, V104, P448
[18] MAZZUCCHELLI L, 1994, AM J PATHOL, V144, P997
[19] Homology of Cytokine Synthesis Inhibitory Factor (IL-10) to the Epstein-Barr Virus Gene BCRFI
Moore, Kevin W.
Vieira, Paulo
Fiorentino, David F.
Trounstine, Mary L.
Khan, Tariq A.
Mosmann, Timothy R.
[J]. JOURNAL OF IMMUNOLOGY, 2012, 189 (05) : 1230 - 1234
[20] TH1-CELL AND TH2-CELL - DIFFERENT PATTERNS OF LYMPHOKINE SECRETION LEAD TO DIFFERENT FUNCTIONAL-PROPERTIES
MOSMANN, TR
COFFMAN, RL
[J]. ANNUAL REVIEW OF IMMUNOLOGY, 1989, 7 : 145 - 173

← 1 2 3 4 →