Stacking and Sweeping in Cyclodextrin-Modified MEKC for Chiral Separation of Hexaconazole, Penconazole and Myclobutanil

被引:22
作者
Ibrahim, Wan Aini Wan [1 ,2 ]
Hermawan, Dadan [1 ,3 ]
Sanagi, M. Marsin [1 ,2 ]
Aboul-Enein, Hassan Y. [1 ,4 ]
机构
[1] Univ Teknol Malaysia, Separat Sci & Technol Grp, Ibnu Sina Inst Fundamental Sci Studies, Utm Skudai 81310, Johor, Malaysia
[2] Univ Teknol Malaysia, Dept Chem, Fac Sci, Utm Skudai 81310, Johor, Malaysia
[3] Univ Jenderal Soedirman UNSOED, Dept Chem, Fac Sci & Engn, Purwokerto, Jawa Tengah, Indonesia
[4] Natl Res Ctr, Pharmaceut & Med Chem Dept, Cairo 12311, Egypt
关键词
Micellar electrokinetic chromatography; Stacking-CD-MEKC; Sweeping-CD-MEKC; Chiral separation; Triazole fungicides; MICELLAR ELECTROKINETIC CHROMATOGRAPHY; SUPERCRITICAL-FLUID CHROMATOGRAPHY; SAMPLE PRECONCENTRATION TECHNIQUES; CAPILLARY CHROMATOGRAPHY; ONLINE PRECONCENTRATION; TRIAZOLE PESTICIDES; BILE-SALTS; TRIADIMENOL; ELECTROPHORESIS; ENANTIOMERS;
D O I
10.1365/s10337-009-1427-y
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A CD-MEKC method for the on-line preconcentration and chiral separation of three chiral triazole fungicides, namely hexaconazole, penconazole and myclobutanil is reported. Simultaneous enantioseparation of the three triazole fungicides was successfully achieved by the CD-MEKC system containing 40 mM HP-gamma-CD + 50 mM SDS in 25 mM phosphate buffer (pH 3.0) solution. Stacking with a reverse migrating micelle (SRMM) and sweeping were then used in this study as two on-line preconcentration methods to enhance the concentration sensitivity. The results indicate that sweeping-CD-MEKC is superior to SRMM-CD-MEKC in terms of the sensitivity enhancement factor (SEF) of the three triazole fungicides ranging from 62- to 67-fold. This is the first report on the chiral separation of hexaconazole, penconazole and myclobutanil by SRMM-CD-MEKC and sweeping-CD-MEKC methods.
引用
收藏
页码:305 / 309
页数:5
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