Homogeneous Analysis: Label-Free and Substrate-Free Aptasensors

被引：53

作者：

Li, Bingling ^{[1
]}

Dong, Shaojun ^{[1
]}

Wang, Erkang ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China

来源：

CHEMISTRY-AN ASIAN JOURNAL | 2010年 / 5卷 / 06期

基金：

中国国家自然科学基金;

关键词：

aptamers; biosensors; DNA structures; fluorescent probes; G-quadruplexes; FREE COLORIMETRIC DETECTION; UNMODIFIED GOLD NANOPARTICLES; G-QUADRUPLEX APTAMERS; DNA APTAMER; PEROXIDASE-ACTIVITY; VISUAL DETECTION; LOGIC GATES; DNAZYME; SENSOR; BINDING;

D O I：

10.1002/asia.200900660

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this Focus Review, we introduce a kind of "label-free" and "substrate-free" (LFSF) aptasensor that carries out the whole sensing process in a homogeneous solution. This means that commonly used covalent label; separation, and immobilization steps in biosensors are successfully avoided, which simplifies the sensing operations to the greatest degree. After brief description about the advantages of aptamers and "LFSF" aptasensors, the main content of the review is divided into fluorescent aptasenors, calorimetric aptasensors, and hemin-aptamer-DNAzyme "LFSF" aptasensors, which are three most widely developed sensing systems in this field. It is hoped that this review can provide an overall scene about how aptamers function as ideal recognition elements in smart analysis.

引用

页码：1262 / 1272

页数：11

共 84 条

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