Gene electrotransfer results in a high-level transduction of rat skeletal muscle and corrects anemia of renal failure

被引:68
作者
Rizzuto, G [1 ]
Cappelletti, M [1 ]
Mennuni, C [1 ]
Wiznerowicz, M [1 ]
DeMartis, A [1 ]
Maione, D [1 ]
Ciliberto, G [1 ]
La Monica, N [1 ]
Fattori, E [1 ]
机构
[1] Ist Ric Biol Mol P Angeletti, I-00040 Rome, Italy
关键词
D O I
10.1089/10430340050129503
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We have investigated the efficacy of a gene transfer strategy based on plasmid DNA electroinjection for the correction of anemia associated with renal failure. An expression plasmid encoding the rat erythropoietin (EPO) cDNA under the control of the CMV promoter as constructed and utilized for this work. Electroinjection of pCMV/rEPO in different rat muscles yielded sustained and long-term EPO production and secretion. The muscle-produced EPO corrected the anemia in five of six nephrectomized rats, used as a model of renal failure. The efficiency of muscle transduction was comparable in rats and mice injected with equivalent amounts of DNA per kilogram of body weight. These results demonstrate that gene electrotransfer can be applied to produce therapeutically significant levels of erythropoietin in chronic renal failure.
引用
收藏
页码:1891 / 1900
页数:10
相关论文
共 53 条
  • [21] KAWAMURA A, 1990, Biotherapy (Dordrecht), V2, P77, DOI 10.1007/BF02172079
  • [22] MECHANISM OF HYPOALBUMINEMIA IN THE 7/8-NEPHRECTOMIZED RAT WITH CHRONIC-RENAL-FAILURE
    KAYSEN, GA
    WATSON, JB
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1982, 243 (04): : F372 - F378
  • [23] KERR DN, 1979, CECIL TXB MED, P1351
  • [24] Gene delivery to skeletal muscle results in sustained expression and systemic delivery of a therapeutic protein
    Kessler, PD
    Podsakoff, GM
    Chen, XJ
    McQuiston, SA
    Colosi, PC
    Matelis, LA
    Kurtzman, GJ
    Byrne, BJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (24) : 14082 - 14087
  • [25] HORMONAL MODULATION OF A GENE INJECTED INTO RAT-HEART INVIVO
    KITSIS, RN
    BUTTRICK, PM
    MCNALLY, EM
    KAPLAN, ML
    LEINWAND, LA
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (10) : 4138 - 4142
  • [26] Safe and effective regulation of hematocrit by gene gun administration of an erythropoietin-encoding DNA plasmid
    Klinman, DM
    Conover, J
    Leiden, JM
    Rosenberg, AS
    Sechler, JMG
    [J]. HUMAN GENE THERAPY, 1999, 10 (04) : 659 - 665
  • [27] Kreiss P, 1999, J GENE MED, V1, P245, DOI 10.1002/(SICI)1521-2254(199907/08)1:4<245::AID-JGM49>3.0.CO
  • [28] 2-G
  • [29] Prolonged expression and effective readministration of erythropoietin delivered with a fully deleted adenoviral vector
    Maione, D
    Wiznerowicz, M
    Delmastro, P
    Cortese, R
    Ciliberto, G
    La Monica, N
    Savino, R
    [J]. HUMAN GENE THERAPY, 2000, 11 (06) : 859 - 868
  • [30] MALGOR LA, 1986, EXP HEMATOL, V14, P250