Quantitative analysis of Tenecteplase in rat plasma samples using LC-MS/MS as an alternative for ELISA

被引：14

作者：

Buscher, B. A. P. ^{[1
]}

Gerritsen, H. ^{[1
]}

van Scholl, I. ^{[1
]}

Cnubben, N. H. P. ^{[1
]}

Brull, L. P. ^{[1
]}

机构：

[1] TNO Qual Life, Business Unit Analyt Res, NL-3700 AJ Zeist, Netherlands

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 852卷 / 1-2期

关键词：

quantitative analysis; proteins; plasma; digestion; liquid chromatography; mass spectrometry;

D O I：

10.1016/j.jchromb.2006.12.053

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An LC-MS/MS method has been developed for the quantitative determination of a protein drug (Tenecteplase; Mw 58,777 Da) in rat plasma. The protein was digested with trypsin without prior clean-up of the plasma sample, without the use of a label nor internal standard. A limited validation was performed to assess the linearity, the sensitivity and the specificity of the method. In addition, the developed method was applied to the quantitative analysis of Tenecteplase in rat plasma samples originating from a single-dose study in rats. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：631 / 634

页数：4

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