Highly Sequence-Dependent Formation of Fluorescent Silver Nanoclusters in Hybridized DNA Duplexes for Single Nucleotide Mutation Identification

被引：444

作者：

Guo, Weiwei

Yuan, Jipei

Dong, Qingzhe

Wang, Erkang ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 03期

基金：

中国国家自然科学基金;

关键词：

AG NANOCLUSTERS; QUANTUM DOTS; IN-VITRO; FLUOROPHORES; TEMPLATE; CLUSTERS; HELIX;

D O I：

10.1021/ja907075s

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Fluorescent silver nanoclusters were successfully synthesized using hybridized DNA duplexes as capping scaffolds. The formation of these emitters was highly sequence-dependent and could specifically identify a single nucleotide mutation, the sickle cell anemia gene mutation. Furthermore, the identification of single-nucleotide differences using this strategy was extended to more general types of single-nucleotide mismatches.

引用

页码：932 / +

页数：5

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