Technical Challenges in Using Human Induced Pluripotent Stem Cells to Model Disease

被引：299

作者：

Saha, Krishanu ^{[1
]}

Jaenisch, Rudolf ^{[1
,2
]}

机构：

[1] Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

[2] MIT, Dept Biol, Cambridge, MA 02142 USA

来源：

CELL STEM CELL | 2009年 / 5卷 / 06期

关键词：

HUMAN SOMATIC-CELLS; IN-VITRO DIFFERENTIATION; HUMAN IPS CELLS; LENTIVIRAL VECTOR; PHYSIOLOGICAL-PARAMETERS; GENETIC-MODIFICATION; LABORATORY-ANIMALS; MOTOR-NEURONS; MOUSE MODEL; GENERATION;

D O I：

10.1016/j.stem.2009.11.009

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Reprogramming of human somatic cells uses readily accessible tissue, such as skin or blood, to generate embryonic-like induced pluripotent stem cells (iPSCs). This procedure has been applied to somatic cells from patients who are classified into a disease group, thus creating "disease-specific" iPSCs. Here, we examine the challenges and assumptions in creating a disease model from a single cell of the patient. Both the kinetics of disease onset and progression as well as the spatial localization of disease in the patient's body are challenges to disease modeling. New tools in genetic modification, reprogramming, biomaterials, and animal models can be used for addressing these challenges.

引用

收藏

页码：584 / 595

页数：12

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