Requirements for bone marrow-derived antigen-presenting cells in priming cytotoxic T cell responses to intracellular pathogens

被引:82
作者
Lenz, LL
Butz, EA
Bevan, MJ
机构
[1] Univ Washington, Sch Med, Dept Immunol, Seattle, WA 98195 USA
[2] Univ Washington, Sch Med, Howard Hughes Med Inst, Seattle, WA 98195 USA
关键词
cross-presentation; cytotoxic T cell; bacterial immunity; viral immunity; radiation chimera;
D O I
10.1084/jem.192.8.1135
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Bone marrow (BM)-derived antigen-presenting cells (APCs) are potent stimulators of T cell immune responses. We investigated the requirements for antigen presentation by these cells in priming cytotoxic T lymphocyte (CTL) responses to intracellular bacterial and viral pathogens. [Parent-->F-1] radiation BM chimeras were constructed using C57BL/6 donors and (C57BL/6 X BALB/c)F-1 recipients. Infection of chimeric mice with either Listeria monocytogenes or vaccinia virus expressing the nucleoprotein (NP) antigen from lymphocytic choriomeningitis virus (LCMV) primed H2-D-b-restricted, but not H2-K-d-restricted CTL responses, demonstrating the requirement for BM-derived APCs for successful priming of CTL responses to these pathogens. Surprisingly, this did not hold true for chimeric mice infected with LCMV itself. LCMV-infected animals developed strong CTL responses specific for both H2-D-b- and H2-L-d-restricted NP epitopes. These findings indicate that in vivo priming of CTL responses to LCMV is remarkably insensitive to deficiencies in antigen presentation by professional BM-derived APCs.
引用
收藏
页码:1135 / 1142
页数:8
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