Recoiling DNA molecule: simulation and experiment

被引：5

作者：

Coelho Neto, J ^{[1
]}

Dickman, R ^{[1
]}

Mesquita, ON ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Dept Fis, ICEx, BR-30123970 Belo Horizonte, MG, Brazil

来源：

PHYSICA A-STATISTICAL MECHANICS AND ITS APPLICATIONS | 2005年 / 345卷 / 1-2期

关键词：

DNA; single molecule; persistence length; recoil dynamics;

D O I：

10.1016/j.physa.2004.07.029

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

Single molecule DNA experiments often generate data from force versus extension measurements involving the tethering of a microsphere to one end of a single DNA molecule while the other is attached to a substrate. We show that the persistence length of single DNA molecules can also be measured based on the recoil dynamics of these DNA-microsphere complexes if appropriate corrections are made to the friction coefficient of the microsphere in the vicinity of the substrate. Comparison between computer simulated recoil curves, generated from the corresponding Langevin equation, and experimental recoils are used to assure the validity of data analysis. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：173 / 184

页数：12

共 20 条

[1] pH-dependent specific binding and combing of DNA [J].

Allemand, JF ;

Bensimon, D ;

Jullien, L ;

Bensimon, A ;

Croquette, V .

BIOPHYSICAL JOURNAL, 1997, 73 (04) :2064-2070

[2] Optical trapping and manipulation of neutral particles using lasers [J].

Ashkin, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (10) :4853-4860

[3] Relaxation dynamics of semiflexible polymers [J].

Bohbot-Raviv, Y ;

Zhao, WZ ;

Feingold, M ;

Wiggins, CH ;

Granek, R .

PHYSICAL REVIEW LETTERS, 2004, 92 (09) :098101-1

[4] Estimating the persistence length of a worm-like chain molecule from force-extension measurements [J].

Bouchiat, C ;

Wang, MD ;

Allemand, JF ;

Strick, T ;

Block, SM ;

Croquette, V .

BIOPHYSICAL JOURNAL, 1999, 76 (01) :409-413

[5] THE SLOW MOTION OF A SPHERE THROUGH A VISCOUS FLUID TOWARDS A PLANE SURFACE [J].

BRENNER, H .

CHEMICAL ENGINEERING SCIENCE, 1961, 16 (3-4) :242-251

[6] ENTROPIC ELASTICITY OF LAMBDA-PHAGE DNA [J].

BUSTAMANTE, C ;

MARKO, JF ;

SIGGIA, ED ;

SMITH, S .

SCIENCE, 1994, 265 (5178) :1599-1600

[7]

Doi M., 1986, THEORY POLYM DYNAMIC

[8]

Faxen H, 1924, Ark. Mat. Astron. Fys., V18, P1

[9] Single-molecule studies of DNA and DNA-protein interactions [J].

Feingold, M .

PHYSICA E, 2001, 9 (03) :616-620

[10] WALL-DRAG EFFECT ON DIFFUSION OF COLLOIDAL PARTICLES NEAR SURFACES - A PHOTON-CORRELATION STUDY [J].

FEITOSA, MIM ;

MESQUITA, ON .

PHYSICAL REVIEW A, 1991, 44 (10) :6677-6685

← 1 2 →