Emergence of extended-spectrum-β-lactamase (CTX-M-9)-producing multiresistant strains of Salmonella enterica serotype Virchow in poultry and humans in France

被引:124
作者
Weill, FX
Lailler, R
Praud, K
Kérouanton, A
Fabre, L
Brisabois, A
Grimont, PAD
Cloeckaert, A
机构
[1] Inst Pasteur, Unite Biodivers Bacteries Pathogenes Emergentes, Ctr Natl Reference Salmonella, INSERM U389, F-75724 Paris, France
[2] Agence Francaise Secur Sanitaire Aliments, Unite Caracterisat & Epidemiol Bacterienne, Maisons Alfort, France
[3] INRA, Unite BioAgresseurs Sante & Environm, F-37380 Nouzilly, France
关键词
D O I
10.1128/JCM.42.12.5767-5773.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
During 2002 to 2003, eight Salmonella enterica serotype Virchow poultry and poultry product isolates from various sources (chicken farms, poultry slaughterhouse, or retail store) and one S. enterica rough strain isolated from human feces were found to produce extended-spectrum P-lactamase CTX-M-9. Poultry and poultry product isolates were recovered from different locations in the southwest of France. The human rough isolate had sequences of flagellin genes (fliC and fljB) typical of serotype Virchow and ribotyping and pulsed-field gel electrophoresis (PFGE) patterns closely similar to those of serotype Virchow strains. PFGE confirmed the clonal relationship between the poultry isolates, while the human isolate displayed a pattern with 94% homology. The bla(CTX-M-9) gene was located on a conjugative plasmid and was shown to be linked to orf513. Plasmid profiling found a very similar EcoRI restriction pattern in six transconjugants studied, including transconjugants obtained from the human isolate. A single hatchery, supplying chicks to the six farms, was identified. Emergence of extended-spectrum beta-lactamase-producing S. enterica strains in food animals is a major concern, as such strains could disseminate on a large scale and lead to antibiotic therapy difficulties.
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页码:5767 / 5773
页数:7
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