Laser sources in direct-view-scanning, tandem-scanning, or Nipkow-disk-scanning confocal microscopy

被引：10

作者：

Fewer, DT ^{[1
]}

Hewlett, SJ ^{[1
]}

McCabe, EM ^{[1
]}

机构：

[1] Univ Dublin Trinity Coll, Dept Phys, Dublin 2, Ireland

来源：

APPLIED OPTICS | 1998年 / 37卷 / 02期

关键词：

D O I：

10.1364/AO.37.000380

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Laser sources offer a potentially low-cost means of improving the light throughput in tandem-scanning confocal microscopy because of their high beam directionality. We measure and compare the optical sectioning characteristics of the tandem-scanning microscope (TSM) employing (i) the traditional choice of incoherent light from a Xe are lamp and (ii) a cited alternative-coherent light from a He-Ne laser source. In general the laser source is found to result in axial responses with pronounced sidelobes, the sizes and locations of which are extremely sensitive to the alignment of the pinhole array. The implications of these results for practical TSM systems are discussed. (C) 1998 Optical Society of America.

引用

页码：380 / 385

页数：6

共 17 条

[1]

Abramowitz M., 1965, HDB MATH FUNCTIONS, P358

[2] FLUORESCENCE IN THE TANDEM SCANNING MICROSCOPE [J].

BOYDE, A ;

JONES, SJ ;

TAYLOR, ML ;

WOLFE, LA ;

WATSON, TF .

JOURNAL OF MICROSCOPY, 1990, 157 :39-49

[3]

Boyde A., 1985, P ROY MICROSC SOC, V20, P131

[4] DEPTH RESPONSE OF CONFOCAL OPTICAL MICROSCOPES [J].

CORLE, TR ;

CHOU, CH ;

KINO, GS .

OPTICS LETTERS, 1986, 11 (12) :770-772

[5]

ELLIS G W, 1979, Journal of Cell Biology, V83, p303A

[6] Direct-view microscopy: Experimental investigation of the dependence of the optical sectioning characteristics on pinhole-array configuration [J].

Fewer, DT ;

Hewlett, SJ ;

McCabe, EM ;

Hegarty, J .

JOURNAL OF MICROSCOPY-OXFORD, 1997, 187 :54-61

[7]

HARD R, 1977, J CELL SCI, V23, P335

[8] Influence of source coherence and aperture distribution on the imaging properties in direct-view microscopy [J].

Hewlett, SJ ;

Fewer, DT ;

McCabe, EM .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 1997, 14 (05) :1066-1075

[9]

MASTERS BR, 1990, CONFOCAL MICROSCOPY, P305

[10] ABERRATION MEASUREMENT BY CONFOCAL INTERFEROMETRY [J].

MATTHEWS, HJ ;

HAMILTON, DK ;

SHEPPARD, CJR .

JOURNAL OF MODERN OPTICS, 1989, 36 (02) :233-250

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