Removal of deaminated cytosines and detection of in vivo methylation in ancient DNA

被引：282

作者：

Briggs, Adrian W. ^{[1
]}

Stenzel, Udo ^{[1
]}

Meyer, Matthias ^{[1
]}

Krause, Johannes ^{[1
]}

Kircher, Martin ^{[1
]}

Paeaebo, Svante ^{[1
]}

机构：

[1] Max Planck Inst Evolutionary Anthropol, D-04103 Leipzig, Germany

来源：

NUCLEIC ACIDS RESEARCH | 2010年 / 38卷 / 06期

关键词：

NEANDERTHAL GENOMIC DNA; MISCODING LESIONS; ENZYMATIC AMPLIFICATION; MITOCHONDRIAL GENOME; ESCHERICHIA-COLI; SEQUENCES; DAMAGE; EXTRACTION; RETRIEVAL; PATTERNS;

D O I：

10.1093/nar/gkp1163

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA sequences determined from ancient organisms have high error rates, primarily due to uracil bases created by cytosine deamination. We use synthetic oligonucleotides, as well as DNA extracted from mammoth and Neandertal remains, to show that treatment with uracil-DNA-glycosylase and endonuclease VIII removes uracil residues from ancient DNA and repairs most of the resulting abasic sites, leaving undamaged parts of the DNA fragments intact. Neandertal DNA sequences determined with this protocol have greatly increased accuracy. In addition, our results demonstrate that Neandertal DNA retains in vivo patterns of CpG methylation, potentially allowing future studies of gene inactivation and imprinting in ancient organisms.

引用

页码：e87.1 / e87.12

页数：12

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