Crystallization and preliminary X-ray study of human dipeptidyl peptidase IV (DPPIV)

被引：10

作者：

Hiramatsu, H

Kyono, K

Shima, H

Fukushima, C

Sugiyama, S

Inaka, K

Yamamoto, A

Shimizu, R

机构：

[1] Tanabe Seiyaku Co Ltd, Discovery Res Lab, Yodogawa Ku, Osaka 5328505, Japan

[2] Maruwa Food Ind Inc, Nara 6391123, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2003年 / 59卷

关键词：

D O I：

10.1107/S0907444903001525

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Human DPPIV has been expressed in the baculovirus system and purified and crystallized using the hanging-drop method. A crystal was obtained from 180 mM Gly-NaOH buffer pH 9.5 containing 18% PEG 4000 and 180 mM sodium acetate. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 118.04, b = 125.92, c = 136.84 Angstrom, and diffracts beyond 2.6 Angstrom resolution. There are two molecules per asymmetric unit, indicating a solvent content of 57.6%.

引用

收藏

页码：595 / 596

页数：2

相关论文

共 18 条

[1]

Binding to human dipeptidyl peptidase IV by adenosine deaminase and antibodies that inhibit ligand binding involves overlapping, discontinuous sites on a predicted β propeller domain [J].

Abbott, CA ;

McCaughan, GW ;

Levy, MT ;

Church, WB ;

Gorrell, MD .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 266 (03) :798-810

[2]

TRIGGERING OF THE PROTEINASE DIPEPTIDYL PEPTIDASE-IV (CD26) AMPLIFIES HUMAN LYMPHOCYTE-T PROLIFERATION [J].

BEDNARCZYK, JL ;

CARROLL, SM ;

MARIN, C ;

MCINTYRE, BW .

JOURNAL OF CELLULAR BIOCHEMISTRY, 1991, 46 (03) :206-218

[3]

Proline specific peptidases [J].

Cunningham, DF ;

O'Connor, B .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1997, 1343 (02) :160-186

[4]

Expression, purification, and characterization of human dipeptidyl peptidase IV/CD26 in Sf9 insect cells [J].

Dobers, J ;

Zimmermann-Kordmann, M ;

Leddermann, M ;

Schewe, T ;

Reutter, W ;

Fan, H .

PROTEIN EXPRESSION AND PURIFICATION, 2002, 25 (03) :527-532

[5]

A NOVEL FORM OF DIPEPTIDYLPEPTIDASE-IV FOUND IN HUMAN SERUM - ISOLATION, CHARACTERIZATION, AND COMPARISON WITH T-LYMPHOCYTE MEMBRANE DIPEPTIDYLPEPTIDASE-IV (CD26) [J].

DUKECOHAN, JS ;

MORIMOTO, C ;

ROCKER, JA ;

SCHLOSSMAN, SF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (23) :14107-14114

[6]

DIPEPTIDYLPEPTIDASE-IV AND TRYPSIN-LIKE ENZYMATIC DEGRADATION OF HUMAN GROWTH-HORMONE RELEASING HORMONE IN PLASMA [J].

FROHMAN, LA ;

DOWNS, TR ;

HEIMER, EP ;

FELIX, AM .

JOURNAL OF CLINICAL INVESTIGATION, 1989, 83 (05) :1533-1540

[7]

β propellers:: structural rigidity and functional diversity [J].

Fülöp, V ;

Jones, DT .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1999, 9 (06) :715-721

[8]

Structures of prolyl oligopeptidase substrate/inhibitor complexes -: Use of inhibitor binding for titration of the catalytic histidine residue [J].

Fülöp, V ;

Szeltner, Z ;

Renner, V ;

Polgár, L .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (02) :1262-1266

[9]

Prolyl oligopeptidase:: An unusual β-propeller domain regulates proteolysis [J].

Fülöp, V ;

Böcskei, Z ;

Polgár, L .

CELL, 1998, 94 (02) :161-170

[10]

Novel sequences propel familiar folds [J].

Jawad, Z ;

Paoli, M .

STRUCTURE, 2002, 10 (04) :447-454