Permeabilizing action of polyethyleneimine on Salmonella typhimurium involves disruption of the outer membrane and interactions with lipopolysaccharide

被引:90
作者
Helander, IM
Latva-Kala, K
Lounatmaa, K
机构
[1] VTT Biotechnol & Food Res, FIN-02044 Espoo, Finland
[2] Univ Helsinki, Inst Biotechnol, FIN-00014 Helsinki, Finland
来源
MICROBIOLOGY-UK | 1998年 / 144卷
关键词
polyethyleneimine; Gram-negative bacteria; outer membrane; lipopolysaccharide; Salmonella typhimurium;
D O I
10.1099/00221287-144-2-385
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Polyethyleneimine (PEI), a polycationic polymer substance used in various bioprocesses as a flocculating agent and to immobilize enzymes, was recently shown to make Gram-negative bacteria permeable to hydrophobic antibiotics and to detergents. Because this suggests impairment of the protective function of the outer membrane (OM), the effect of PEI on the ultrastructure of Salmonella typhimurium was investigated. Massive alterations in the OM of PEI-treated and thin-sectioned bacteria were observed by electron microscopy. Vesicular structures were seen on the surface of the OM, but no liberation of the membrane or its fragments was evident. Since a potential mechanism for the action of PEI could be its binding to anionic LPSs on the OM surface, the interaction of PEI with isolated LPSs was assayed in vitro. The solubility of smooth-type LPSs of Salmonella, regardless of the sugar composition of their O-specific chains, was not affected by PEI, nor was that of Ra-LPS (lacking O-specific chains but having a complete core oligosaccharide). PEI strongly decreased the solubility of rough-type LPSs of the chemotypes Rb2 and Re, whereas it had only a weak effect on the abnormally cationic Rb2-type pmrA mutant LPS, suggesting that the negative charge to mass ratio of LPS plays a critical role in the interaction.
引用
收藏
页码:385 / 390
页数:6
相关论文
共 25 条
[1]  
[Anonymous], ENCY MOL BIOL MOL ME
[2]   ELECTRODIALYSIS OF LIPOPOLYSACCHARIDES AND THEIR CONVERSION TO UNIFORM SALT FORMS [J].
GALANOS, C ;
LUDERITZ, O .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1975, 54 (02) :603-610
[3]   A NEW METHOD FOR EXTRACTION OF R-LIPOPOLYSACCHARIDES [J].
GALANOS, C ;
LUDERITZ, O ;
WESTPHAL, O .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1969, 9 (02) :245-&
[4]   SEPARATION AND CHARACTERIZATION OF 2 CHEMICALLY DISTINCT LIPOPOLYSACCHARIDES IN 2 PECTINATUS SPECIES [J].
HELANDER, IM ;
HURME, R ;
HAIKARA, A ;
MORAN, AP .
JOURNAL OF BACTERIOLOGY, 1992, 174 (10) :3348-3354
[5]   Polyethyleneimine is an effective permeabilizer of Gram-negative bacteria [J].
Helander, IM ;
Alakomi, HL ;
LatvaKala, K ;
Koski, P .
MICROBIOLOGY-UK, 1997, 143 :3193-3199
[6]   INCREASED SUBSTITUTION OF PHOSPHATE GROUPS IN LIPOPOLYSACCHARIDES AND LIPID-A OF THE POLYMYXIN-RESISTANT PMRA MUTANTS OF SALMONELLA-TYPHIMURIUM - A P-31-NMR STUDY [J].
HELANDER, IM ;
KILPELAINEN, I ;
VAARA, M .
MOLECULAR MICROBIOLOGY, 1994, 11 (03) :481-487
[7]   STRUCTURAL STUDIES ON O-SPECIFIC SIDE-CHAINS OF CELL-WALL LIPOPOLYSACCHARIDE FROM SALMONELLA TYPHIMURIUM LT2 [J].
HELLERQV.CG ;
LINDBERG, B ;
SVENSSON, S ;
HOLME, T ;
LINDBERG, AA .
CARBOHYDRATE RESEARCH, 1969, 9 (02) :237-&
[8]   STRUCTURAL STUDIES OF THE O-ANTIGEN POLYSACCHARIDE OF SALMONELLA-THOMPSON, SEROGROUP-C1 (6,7) [J].
LINDBERG, B ;
LEONTEIN, K ;
LINDQUIST, U ;
SVENSON, SB ;
WRANGSELL, G ;
DELL, A ;
ROGERS, M .
CARBOHYDRATE RESEARCH, 1988, 174 :313-322
[9]  
Lounatmaa K, 1985, ENTEROBACTERIAL SURF, P243
[10]   SELECTIVE FLOCCULATION OF NUCLEIC-ACIDS, LIPIDS, AND COLLOIDAL PARTICLES FROM A YEAST-CELL HOMOGENATE BY POLYETHYLENEIMINE, AND ITS SCALE-UP [J].
MILBURN, P ;
BONNERJEA, J ;
HOARE, M ;
DUNNILL, P .
ENZYME AND MICROBIAL TECHNOLOGY, 1990, 12 (07) :527-532