Amperometric hydrogen peroxide biosensor based on horseradish peroxidase-labeled nano-Au colloids immobilized on poly(2,6-pyridinedicarboxylic acid) layer by cysteamine

被引:10
作者
Li, CX [1 ]
Deng, KQ
Shen, GL
Yu, RQ
机构
[1] Hunan Univ Sci & Technol, Coll Chem & Chem Engn, Xiangtan 411201, Peoples R China
[2] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China
关键词
D O I
10.2116/analsci.20.1277
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A sensitive hydrogen peroxidase (H2O2) amperometric sensor based on horseradish peroxidase (HRP)-labeled nano-Au colloids has been proposed. Nano-Au colloids were immobilized by the thiol group of cysteamine, which was associated with the carboxyl groups of poly(2,6-pyridinedicarboxylic acid) (PPDA). With the aid of the hydroquinone, the sensor displayed excellent electrocatalytical response to the reduction of H2O2, Compared with the non-Au-colloid modified electrode, i.e., PPDA/HRP, the Au-colloid modified electrode exhibited better performance characteristics, including stability, reproducibility, sensitivity and accuracy. The biosensor shows a linear response to H2O2 in the range of 3.0 x 10(-7) - 2 x 10(-3) M. The detection limit was 1.0 X 10(-7) M.
引用
收藏
页码:1277 / 1281
页数:5
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