Gravity-induced convective flow in microfluidic systems: Electrochemical characterization and application to enzyme-linked immunosorbent assay tests

被引：48

作者：

Morier, P ^{[1
]}

Vollet, C ^{[1
]}

Michel, PE ^{[1
]}

Reymond, F ^{[1
]}

Rossier, JS ^{[1
]}

机构：

[1] DiagnoSwiss SA, Cimo SA, CH-1870 Monthey, Switzerland

来源：

ELECTROPHORESIS | 2004年 / 25卷 / 21-22期

关键词：

enzyme-linked immunosorbent assay; gravity-induced convective flow; microfluidic system; miniaturization; thyroid-stimulating hormone;

D O I：

10.1002/elps.200406093

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A way of using gravity flow to induce a linear convection within a microfluidic system is presented. It is shown and mathematically supported that tilting a 1 cm long covered microchannel is enough to generate flow rates up to 1000 nL(.)min(-1), which represents a linear velocity of 2.4 mm(.)s(-1). This paper also presents a method to monitor the microfluidic events occurring in a covered microchannel when a difference of pressure is applied to force a solution to flow in said covered microchannel, thanks to electrodes inserted in the microfluidic device. Gravity-induced flow monitored electrochemically is applied to the performance of a parallel-microchannel enzyme-linked immunosorbent assay (ELISA) of the thyroid-stimulating hormone (TSH) with electrochemical detection. A simple method for generating and monitoring fluid flows is described, which can, for instance, be used for controlling parallel assays in microsystems.

引用

页码：3761 / 3768

页数：8

共 24 条

[1] An accurate microdisc simulation model for recessed microdisc electrodes [J].