Constitutive activity of Sauromatum guttatum alternative oxidase in Schizosaccharomyces pombe implicates residues in addition to conserved cysteines in α-keto acid activation

被引：34

作者：

Crichton, PG ^{[1
]}

Affourtit, C ^{[1
]}

Albury, MS ^{[1
]}

Carré, JE ^{[1
]}

Moore, AL ^{[1
]}

机构：

[1] Univ Sussex, Sch Life Sci, Dept Biochem, Brighton BN1 9QG, E Sussex, England

来源：

FEBS LETTERS | 2005年 / 579卷 / 02期

关键词：

alternative oxidase structure; regulation; pyruvate; plant respiration; S. pombe mitochondria;

D O I：

10.1016/j.febslet.2004.10.107

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Activity of the plant mitochondrial alternative oxidase (AOX) can be regulated by organic acids, notably pyruvate. To date, only two well-conserved cysteine residues have been implicated in this process. We report the functional expression of two AOX isozymes (Sauromatum gattatum Sg-AOX and Arabidopsis thaliana At-AOX1a) in Schizosaccharomyces pombe. Comparison of the response of these two isozymes to pyruvate in isolated yeast mitochondria and disrupted mitochondrial membranes reveals that in contrast to At-AOX1a, Sg-AOX activity is insensitive to pyruvate and appears to be in a constitutively active state. As both of these isozymes conserve the two cysteines, we propose that such contrasting behaviour must be a direct result of differences in their amino acid sequence and have subsequently identified novel candidate residues. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：331 / 336

页数：6

共 33 条

[21] CLONING AND CHARACTERIZATION OF THE RAD9 DNA-REPAIR GENE FROM SCHIZOSACCHAROMYCES POMBE [J].

MURRAY, JM ;

CARR, AM ;

LEHMANN, AR ;

WATTS, FZ .

NUCLEIC ACIDS RESEARCH, 1991, 19 (13) :3525-3531

[22] HIGH-FREQUENCY TRANSFORMATION METHOD AND LIBRARY TRANSDUCING VECTORS FOR CLONING MAMMALIAN CDNAS BY TRANSCOMPLEMENTATION OF SCHIZOSACCHAROMYCES-POMBE [J].

OKAZAKI, K ;

OKAZAKI, N ;

KUME, K ;

JINNO, S ;

TANAKA, K ;

OKAYAMA, H .

NUCLEIC ACIDS RESEARCH, 1990, 18 (22) :6485-6489

[23] Regulation of the cyanide-resistant alternative oxidase of plant mitochondria -: Identification of the cysteine residue involved in α-keto acid stimulation and intersubunit disulfide bond formation [J].

Rhoads, DM ;

Umbach, AL ;

Sweet, CR ;

Lennon, AM ;

Rauch, GS ;

Siedow, JN .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (46) :30750-30756

[24] ISOLATION AND CHARACTERIZATION OF A CDNA CLONE ENCODING AN ALTERNATIVE OXIDASE PROTEIN OF SAUROMATUM-GUTTATUM (SCHOTT) [J].

RHOADS, DM ;

MCINTOSH, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) :2122-2126

[25]

Sambrook J, 1989, MOL CLONING LAB MANU

[26] MEASUREMENT OF PROTEIN USING BICINCHONINIC ACID [J].

SMITH, PK ;

KROHN, RI ;

HERMANSON, GT ;

MALLIA, AK ;

GARTNER, FH ;

PROVENZANO, MD ;

FUJIMOTO, EK ;

GOEKE, NM ;

OLSON, BJ ;

KLENK, DC .

ANALYTICAL BIOCHEMISTRY, 1985, 150 (01) :76-85

[27] COVALENT AND NONCOVALENT DIMERS OF THE CYANIDE-RESISTANT ALTERNATIVE OXIDASE PROTEIN IN HIGHER-PLANT MITOCHONDRIA AND THEIR RELATIONSHIP TO ENZYME-ACTIVITY [J].

UMBACH, AL ;

SIEDOW, JN .

PLANT PHYSIOLOGY, 1993, 103 (03) :845-854

[28] REGULATION OF ALTERNATIVE OXIDASE KINETICS BY PYRUVATE AND INTERMOLECULAR DISULFIDE BOND REDOX STATUS IN SOYBEAN SEEDLING MITOCHONDRIA [J].

UMBACH, AL ;

WISKICH, JT ;

SIEDOW, JN .

FEBS LETTERS, 1994, 348 (02) :181-184

[29] Activation of the plant mitochondrial alternative oxidase:: insights from site-directed mutagenesis [J].

Umbach, AL ;

Gonzàlez-Meler, MA ;

Sweet, CR ;

Siedow, JN .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2002, 1554 (1-2) :118-128

[30] The reaction of the soybean cotyledon mitochondrial cyanide-resistant oxidase with sulfhydryl reagents suggests that alpha-keto acid activation involves the formation of a thiohemiacetal [J].

Umbach, AL ;

Siedow, JN .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (40) :25019-25026

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