Relationships between dopamine-induced changes in cytosolic free calcium concentration ([Ca2+]i) and rate of prolactin secretion -: Elevated [Ca2+]i does not indicate prolactin release

被引:6
作者
Chang, A [1 ]
Shin, SH [1 ]
机构
[1] Queens Univ, Dept Physiol, Kingston, ON K7L 3N6, Canada
关键词
dopamine; cytosolic calcium; prolactin; fluo-3; GH(4)ZR(7);
D O I
10.1007/BF02801329
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
This study was undertaken to investigate the relationship between dopamine (DA) induced changes in the cytosolic calcium concentration ([Ca2+](i)) and the rate of prolactin secretion using GH(4)ZR(7), a rat pituitary cell line, which express only one subtype of D-2 receptor. GH(4)ZR(7) cells were loaded with Fluo-3, a fluorescent Ca2+ indicator, and then perifused with two different doses of DA (10(-7) mol/L and 5 x 10(-4)mol/L). We monitored changes in [Ca2+](i) and rate of prolactin release simultaneously by attaching a spectrofluorometer to a dynamic perifusion system. DA has stimulatory and inhibitory effect on prolactin secretion in GH(4)ZR(7) cells; 10(-7) mol/L DA slightly increased [Ca2+](i) and stimulated prolactin release, whereas 5 x 10(-4) mol/L DA decreased [Ca2+](i) and inhibited prolactin secretion, When the cells were pretreated with pertussis toxin (PTX), 10(-7) mol/L DA had no significant change in [Ca2+](i) while stimulating prolactin release, and 5 x 10(-4) mol/L DA reduced [Ca2+](i) without having any significant effect on the rate of prolactin secretion. The results of this study demonstrate that changes in [Ca2+](i) do not always correlate with the rate of prolactin release from lactotrophs. The dissociation between [Ca2+](i) and prolactin release is somewhat expected considering the diverse role of [Ca2+](i) and post-[Ca2+](i) events, which can change the rate of prolactin release.
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页码:343 / 349
页数:7
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