Determination of cobalamins and cobinamides by microbore reversed-phase HPLC with spectrophotometric, ion-spray ionization MS and inductively coupled plasma MS detection

被引:52
作者
Chassaigne, H [1 ]
Lobinski, R [1 ]
机构
[1] CNRS EP 132, F-64000 Pau, France
关键词
cobalamins; cobinamides; HPLC; ion-spray MS; ICP MS;
D O I
10.1016/S0003-2670(97)00679-X
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Microbore reversed-phase HPLC was optimized for the separation of cyanocobalamin, 5'-desoxyadenosylcobalamin (coenzyme Bit), methylcobalamin, hydroxocobalamin, aquocobalamin and cobinamide dicyanide isoforms. Various detection modes: UV at 278 nm, ion-spray (IS)-MS and direct-injection nebulization ICP-MS were examined and compared. IS-MS allows one to identify, unambiguously, the eluted compounds, whereas ICP MS was found to be more sensitive than any other on-line detection techniques described for the cobalamin analogues, so far allowing to achieve detection limits down to ca. LO ng ml(-1). The usefulness of the methods developed was demonstrated for the determination of cobalamins present in pharmaceutical preparations. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:227 / 235
页数:9
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