Production of extracellular β-glucosidase by Monascus purpureus on different growth substrates

被引:51
作者
Daroit, Daniel J. [1 ]
Silveira, Silvana T. [1 ]
Hertz, Plinho F. [1 ]
Brandelli, Adriano [1 ]
机构
[1] Univ Fed Rio Grande do Sul, Lab Bioquim & Microbiol Aplicada, Dept Ciencia Alimentos ICTA, BR-91501970 Porto Alegre, RS, Brazil
关键词
agro-industrial wastes; cellulase; enzyme; beta-glucosidase; Monascus purpureus; submerged fermentation;
D O I
10.1016/j.procbio.2007.01.012
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Various agro-industrial residues in combination with peptone, NH4Cl and/or soy bran were screened as substrates for extracellular beta-glucosidase (BGL) production by Monascus purpureus NRRL1992 on submerged fermentations (SmF). Higher BGL production was achieved when the agro-industrial residues were combined with peptone, and the utilization of NH4Cl (inorganic nitrogen source) had not supported high enzyme production. The combination between grape waste and peptone was the best for enzyme production, and was selected as the growth substrate for further investigations. The evaluation of the effects of the medium components on enzyme production showed that the influence of peptone was more important than grape waste. The production of extracellular BGL by M. purpureus was inducible and controlled by carbon (glucose) catabolite repression. (C) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:904 / 908
页数:5
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