A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide

被引：126

作者：

Desmarchelier, PM

Bilge, SS

Fegan, N

Mills, L

Vary, JC

Tarr, PI

机构：

[1] Commonwealth Sci & Ind Res Org, Brisbane, Qld, Australia

[2] Univ Washington, Seattle, WA 98195 USA

[3] Childrens Hosp & Med Ctr, Seattle, WA 98105 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1998年 / 36卷 / 06期

关键词：

D O I：

10.1128/JCM.36.6.1801-1804.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A PCR was developed for the detection of Escherichia coli O157 based on the rfbE O-antigen synthesis genes. A 479-bp PCR product was amplified specifically from E. coli O157 in cell lysates containing 200 or 2 CFU following crude DNA extraction. The PCR detected <1 CFU off, coli O157 per mi in raw milk following enrichment.

引用

页码：1801 / 1804

页数：4

共 20 条

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