Matrix metaltoproteinases and their inhibitors in the cervical mucus plug at term of pregnancy

被引：19

作者：

Becher, N ^{[1
]}

Hein, M

Danielsen, CC

Uldbjerg, N

机构：

[1] Univ Aarhus, Skejby Hosp, Lab Obstet & Gynecol, DK-8200 Aarhus N, Denmark

[2] Univ Aarhus, Inst Anat, Dept Connect Tissue Biol, Aarhus, Denmark

来源：

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY | 2004年 / 191卷 / 04期

关键词：

cervical mucus plug; pregnancy; matrix metalloproteinase-2; matrix metalloproteinase-8; matrix metalloproteinase-9; tissue inhibitor of matrix metalloproteinase-1;

D O I：

10.1016/j.ajog.2004.03.023

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: This study was undertaken to evaluate the presence of matrix (MMPs) and their inhibitors in the cervical mucus plug obtained during active labor at term. Study design: Cervical mucus plugs from 17 healthy women in normal active labor were homogenized, extracted, and analyzed by 4 different assays. Results: Gelatin zymography revealed large amounts of MMP-2 and MMP-9, and triple-helical collagen degradation demonstrated collagenase activity (MMP-8). Reverse zymography showed that tissue inhibitor of matrix metalloproteinase I (TIMP-1) and TIMP-2 are detectable in the cervical mucus plug. Quantification by enzyme-linked immunosorbent assay confirmed the presence of MMP-2, MMP-8, MMP-9, and TIMP-1. Conclusion: Our results suggest that proteolytic or nonproteolytic effects of MMP and TIMP may play a part in the function of the cervical mucus plug of pregnancy. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：1232 / 1239

页数：8

共 27 条

[1]

Athayde N, 1999, J Matern Fetal Med, V8, P213

[2] Tissue inhibitors of metalloproteinases: evolution, structure and function [J].

Brew, K ;

Dinakarpandian, D ;

Nagase, H .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2000, 1477 (1-2) :267-283

[3]

Danielsen CC, 1998, J MOL CELL CARDIOL, V30, P1431

[4] DIFFERENCE IN THERMAL-STABILITY OF TYPE-I AND TYPE-III COLLAGEN FROM RAT SKIN [J].

DANIELSEN, CC .

BIOCHEMICAL JOURNAL, 1982, 203 (01) :323-326

[5] Collagenolytic enzymes (gelatinases) and their inhibitors in human amniochorionic membrane [J].

Fortunato, SJ ;

Menon, R ;

Lombardi, SJ .

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1997, 177 (04) :731-741

[6] MMP/TIMP imbalance in amniotic fluid during PROM: an indirect support for endogenous pathway to membrane rupture [J].

Fortunato, SJ ;

Menon, R ;

Lombardi, SJ .

JOURNAL OF PERINATAL MEDICINE, 1999, 27 (05) :362-368

[7] Mechanisms of disease - Intrauterine infection and preterm delivery [J].

Goldenberg, RL ;

Hauth, JC ;

Andrews, WW .

NEW ENGLAND JOURNAL OF MEDICINE, 2000, 342 (20) :1500-1507

[8]

Gomez DE, 1997, EUR J CELL BIOL, V74, P111

[9] Intrauterine infection and prematurity [J].

Gonçalves, LF ;

Chaiworapongsa, T ;

Romero, R .

MENTAL RETARDATION AND DEVELOPMENTAL DISABILITIES RESEARCH REVIEWS, 2002, 8 (01) :3-13

[10] Antimicrobial factors in the cervical mucus plug [J].

Hein, M ;

Valore, EV ;

Helmig, RB ;

Uldbjerg, N ;

Ganz, T .

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2002, 187 (01) :137-144

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