Evaluation of Diagnostic Tests for Clostridium difficile Infection

被引：127

作者：

Swindells, Jonathan ^{[1
]}

Brenwald, Nigel ^{[1
]}

Reading, Nathan ^{[1
]}

Oppenheim, Beryl ^{[1
]}

机构：

[1] City Hosp, Dept Med Microbiol, Birmingham, W Midlands, England

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 02期

关键词：

REAL-TIME PCR; ENZYME-IMMUNOASSAY; TOXIGENIC CULTURE; TOXIN; ALGORITHM; DIARRHEA; ASSAY; CYTOTOXIN; ANTIGEN;

D O I：

10.1128/JCM.01579-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We evaluated toxigenic Clostridium difficile detection by a lateral flow assay for antigen and toxin, an enzyme immunoassay, and two commercial PCR methods. Compared to the cell cytotoxicity neutralization assay and toxigenic culture, both toxin detection methods lacked sensitivity. PCR following combined antigen and toxin detection provided the most useful diagnostic information.

引用

页码：606 / 608

页数：3

共 17 条

[1] A European survey of diagnostic methods and testing protocols for Clostridium difficile [J].

Barbut, F ;

Delmée, M ;

Brazier, JS ;

Petit, JC ;

Poxton, IR ;

Rupnik, M ;

Lalande, V ;

Schneider, C ;

Mastrantonio, P ;

Alonso, R ;

Kuipjer, E ;

Tvede, M .

CLINICAL MICROBIOLOGY AND INFECTION, 2003, 9 (10) :989-996

[2] Laboratory diagnosis of Clostridium difficile associated diarrhoea:: a plea for culture [J].

Delmée, M ;

Van Broeck, J ;

Simon, A ;

Janssens, M ;

Avesani, V .

JOURNAL OF MEDICAL MICROBIOLOGY, 2005, 54 (02) :187-191

[3] Rapid and reliable diagnostic algorithm for detection of Clostridium difficile [J].

Fenner, Lukas ;

Widmer, Andreas F. ;

Goy, Gisela ;

Rudin, Sonja ;

Frei, Reno .

JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (01) :328-330

[4]

FULLER D, 2008, 18 EUR C CLIN MICR I

[5] Is a two-step glutamate dehyrogenase antigen-cytotoxicity neutralization assay algorithm superior to the premier toxin a and B enzyme immunoassay for laboratory detection of Clostridium difficile? [J].

Gilligan, Peter H. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (04) :1523-1525

[6]

*HLTH PROT AG, 2008, 10 BSOP HLTH PROT AG

[7] Decreased effectiveness of metronidazole for the treatment of Clostridium difficile infection? [J].

Kuijper, Ed J. ;

Wilcox, Mark H. .

CLINICAL INFECTIOUS DISEASES, 2008, 47 (01) :63-65

[8] Diagnosis of Clostridium difficile infection by toxin detection kits: a systematic review [J].

Planche, Tim ;

Aghaizu, Adamma ;

Holliman, Richard ;

Riley, Peter ;

Poloniecki, Jan ;

Breathnach, Aodhan ;

Krishna, Sanjeev .

LANCET INFECTIOUS DISEASES, 2008, 8 (12) :777-784

[9] Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile [J].

Reller, Megan E. ;

Lema, Clara A. ;

Perl, Trish M. ;

Cai, Mian ;

Ross, Tracy L. ;

Speck, Kathleen A. ;

Carroll, Karen C. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (11) :3601-3605

[10] Performance of the TechLab C DIFF CHEK-60 enzyme immunoassay (EIA) in combination with the C difficile tox A/B II EIA Kit, the triage C difficile panel immunoassay, and a cytotoxin assay for diagnosis of Clostridium difficile-associated diarrhea [J].

Snell, H ;

Ramos, M ;

Longo, S ;

John, M ;

Hussain, Z .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (10) :4863-4865

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