High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples

被引：166

作者：

Hows, MEP

Lacroix, L

Heidbreder, C

Organ, AJ

Shah, AJ

机构：

[1] GlaxoSmithKline, CEDD, I-37135 Verona, Italy

[2] GlaxoSmithKline, I-37135 Verona, Italy

来源：

JOURNAL OF NEUROSCIENCE METHODS | 2004年 / 138卷 / 1-2期

关键词：

dopamine; 5-hydroxytryptamine; norepinephrine; cocaine; RP-HPLC; tandem mass spectrometry; microdialysis;

D O I：

10.1016/j.jneumeth.2004.03.021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been developed for the measurement of dopamine (DA), 5-hydroxytryptamine (5HT) and norepinephrine (NE) in brain microdialy sates. The assay has also been utilised for the simultaneous measurement of these neurotransmitters and cocaine in brain dialysates. The neurotransmitters and cocaine were resolved in a single 4-min run using a binary gradient elution profile. The analytes were detected using tandem mass spectrometry in the positive ion electrospray mode. The limits of detection for DA, NE, 5HT and cocaine were 200, 1000, 900 pM and 1 pg ml(-1), respectively. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：123 / 132

页数：10

共 20 条

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