Fluorescence resonance energy transfer as a structural tool for nucleic acids

被引:124
作者
Lilley, DMJ [1 ]
Wilson, TJ [1 ]
机构
[1] Univ Dundee, Dept Biochem, CRC, Nucl Acid Struct Res Grp, Dundee DD1 4HN, Scotland
关键词
D O I
10.1016/S1367-5931(00)00124-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fluorescence resonance energy transfer is a spectroscopic method that provides distance information on macromolecules in solution in the range 20-80 Angstrom. It is particularly suited to the analysis of the global structure of nucleic acids because the long-range distance information provides constraints when modelling these important structures. The application of fluorescence resonance energy transfer to nucleic acid structure has seen a resurgence of interest in the past decade, which continues to increase. An especially exciting development is the recent extension to single-molecule studies.
引用
收藏
页码:507 / 517
页数:11
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