Fluorescence resonance energy transfer as a structural tool for nucleic acids

被引：124

作者：

Lilley, DMJ ^{[1
]}

Wilson, TJ ^{[1
]}

机构：

[1] Univ Dundee, Dept Biochem, CRC, Nucl Acid Struct Res Grp, Dundee DD1 4HN, Scotland

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2000年 / 4卷 / 05期

关键词：

D O I：

10.1016/S1367-5931(00)00124-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence resonance energy transfer is a spectroscopic method that provides distance information on macromolecules in solution in the range 20-80 Angstrom. It is particularly suited to the analysis of the global structure of nucleic acids because the long-range distance information provides constraints when modelling these important structures. The application of fluorescence resonance energy transfer to nucleic acid structure has seen a resurgence of interest in the past decade, which continues to increase. An especially exciting development is the recent extension to single-molecule studies.

引用

页码：507 / 517

页数：11

共 93 条

[21] *ZWISCHENMOLEKULARE ENERGIEWANDERUNG UND FLUORESZENZ [J].

FORSTER, T .

ANNALEN DER PHYSIK, 1948, 2 (1-2) :55-75

[22] Use of fluorescence resonance energy transfer to investigate the conformation of DNA substrates bound to the Klenow fragment [J].

Furey, WS ;

Joyce, CM ;

Osborne, MA ;

Klenerman, D ;

Peliska, JA ;

Balasubramanian, S .

BIOCHEMISTRY, 1998, 37 (09) :2979-2990

[23] A quantitative method for evaluating the stabilities of nucleic acids [J].

Gelfand, CA ;

Plum, GE ;

Mielewczyk, S ;

Remeta, DP ;

Breslauer, KJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (11) :6113-6118

[24] REAL-TIME KINETICS OF RESTRICTION-ENDONUCLEASE CLEAVAGE MONITORED BY FLUORESCENCE RESONANCE ENERGY-TRANSFER [J].

GHOSH, SS ;

EIS, PS ;

BLUMEYER, K ;

FEARON, K ;

MILLAR, DP .

NUCLEIC ACIDS RESEARCH, 1994, 22 (15) :3155-3159

[25] KINKING OF DNA AND RNA HELICES BY BULGED NUCLEOTIDES OBSERVED BY FLUORESCENCE RESONANCE ENERGY-TRANSFER [J].

GOHLKE, C ;

MURCHIE, AIH ;

LILLEY, DMJ ;

CLEGG, RM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (24) :11660-11664

[26] Severe axial bending of RNA induced by the U1A binding element present in the 3' untranslated region of the U1A mRNA [J].

Grainger, RJ ;

Murchie, AIH ;

Norman, DG ;

Lilley, DMJ .

JOURNAL OF MOLECULAR BIOLOGY, 1997, 273 (01) :84-92

[27] Binding of U1A protein to the 3′ untranslated region of its pre-mRNA [J].

Grainger, RJ ;

Norman, DG ;

Lilley, DMJ .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 288 (04) :585-594

[28] Structure of the polyadenylation regulatory element of the human U1A pre-mRNA 3'-untranslated region and interaction with the U1A protein [J].

Gubser, CC ;

Varani, G .

BIOCHEMISTRY, 1996, 35 (07) :2253-2267

[29] Three mechanistic steps detected by FRET after presynaptic filament formation in homologous recombination. ATP hydrolysis required for release of oligonucleotide heteroduplex product from RecA [J].

Gumbs, OH ;

Shaner, SL .

BIOCHEMISTRY, 1998, 37 (33) :11692-11706

[30] Ligand-induced conformational changes observed in single RNA molecules [J].

Ha, T ;

Zhuang, XW ;

Kim, HD ;

Orr, JW ;

Williamson, JR ;

Chu, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (16) :9077-9082

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