The lactoperoxidase system links anion transport to host defense in cystic fibrosis

被引:91
作者
Conner, Gregory E.
Wijkstrom-Frei, Corinne
Randell, Scott H.
Fernandez, Vania E.
Salathe, Matthias
机构
[1] Univ Miami, Sch Med, Dept Cell Biol & Anat, Miami, FL 33136 USA
[2] Univ Miami, Sch Med, Div Pulm & Crit Care Med, Miami, FL 33152 USA
[3] Univ N Carolina, Cyst Fibrosis Pulm Res & Treatment Ctr, Div Pediat Pulm Med, Chapel Hill, NC 27515 USA
关键词
lactoperoxidase; CFTR; host defense; thiocyanate; hydrogen peroxide; cystic fibrosis;
D O I
10.1016/j.febslet.2006.12.025
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chronic respiratory infections in cystic fibrosis result from CFTR channel mutations but how these impair antibacterial defense is less clear. Airway host defense depends on lactoperoxidase (LPO) that requires thiocyanate (SCN-) to function and epithelia use CFTR to concentrate SCN- at the apical surface. To test whether CFTR mutations result in impaired LPO-mediated host defense, CF epithelial SCN- transport was measured. CF epithelia had significantly lower transport rates and did not accumulate SCN- in the apical compartment. The lower CF [SCN(--)l did not support LPO antibacterial activity. Modeling of airway LPO activity suggested that reduced transport impairs LPO-mediated defense and cannot be compensated by LPO or H2O2 upregulation. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:271 / 278
页数:8
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