Low-level iron-dependent mutants of Listeria monocytogenes and their virulence in macrophages

被引:6
作者
Andre, P
Oberle, S
Specklin, V
Lombard, Y
Vidon, DJM
机构
[1] Univ Strasbourg 1, UFR Sci Pharmaceut, INSERM, U392,Lab Bacteriol & Cryptogamie, F-67401 Illkirch Graffenstaden, France
[2] Univ Strasbourg 1, UFR Sci Pharmaceut, Lab Pathol Commun Entre Cellules Nerveuses & Musc, F-67401 Illkirch Graffenstaden, France
关键词
Listeria monocytogenes; iron; virulence; macrophages;
D O I
10.1139/W03-015
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Listeria monocytogenes is an opportunistic intracellular pathogen capable of growth within phagocytic cells that requires iron for growth and virulence expression. In the presence of an appropriate concentration of tropolone, an iron-chelating agent, growth of L. monocytogenes is completely inhibited. However, this inhibition can be relieved by addition of dopamine, norepinephrine, or ferric citrate. By selection on streptonigrin medium supplemented with tropolone and norepinephrine, we have obtained two spontaneous mutants, Lm-8 and Lm-15, with the same iron dependence but lower iron dependence than the wild-type Lm-B38. The association between iron requirement and virulence of the two mutants and the wild type was studied in the J774 macrophage cell line. One hour after phagocytosis by the J774 macrophage cell line, the two mutants and the parental strain displayed no difference in the number of phagocytosed bacteria. Twenty-four hours after phagocytosis, the number of bacteria within the surviving macrophages was identical for the wild strain and the two clones. However, only 40% of macrophage cells infected with Lm-8 and 90% of those infected with Lm-15 were alive after 24 h in comparison with macrophage cells infected with the parental strain Lm-B38. These data demonstrate that there is no direct correlation between iron requirement and virulence of L monocytogenes in the J774 macrophage cell line.
引用
收藏
页码:78 / 84
页数:7
相关论文
共 33 条
[1]   IRON ACQUISITION-SYSTEMS OF LISTERIA-MONOCYTOGENES [J].
ADAMS, TJ ;
VARTIVARIAN, S ;
COWART, RE .
INFECTION AND IMMUNITY, 1990, 58 (08) :2715-2718
[2]   ROLE OF TRANSFERRIN, TRANSFERRIN RECEPTORS, AND IRON IN MACROPHAGE LISTERICIDAL ACTIVITY [J].
ALFORD, CE ;
KING, TE ;
CAMPBELL, PA .
JOURNAL OF EXPERIMENTAL MEDICINE, 1991, 174 (02) :459-466
[3]   Extracellular iron reductase activity produced by Listeria monocytogenes [J].
Barchini, E ;
Cowart, RE .
ARCHIVES OF MICROBIOLOGY, 1996, 166 (01) :51-57
[4]   Specific binding of the Listeria monocytogenes transcriptional regulator PrfA to target sequences requires additional factor(s) and is influenced by iron [J].
Bockmann, R ;
Dickneite, C ;
Middendorf, B ;
Goebel, W ;
Sokolovic, Z .
MOLECULAR MICROBIOLOGY, 1996, 22 (04) :643-653
[5]   PrfA mediates specific binding of RNA polymerase of Listeria monocytogenes to PrfA-dependent virulence gene promoters resulting in a transcriptionally active complex [J].
Böckmann, R ;
Dickneite, C ;
Goebel, W ;
Bohne, J .
MOLECULAR MICROBIOLOGY, 2000, 36 (02) :487-497
[6]   Interactions between Listeria monocytogenes and host mammalian cells [J].
Braun, L ;
Cossart, P .
MICROBES AND INFECTION, 2000, 2 (07) :803-811
[7]   Iron availability affects entry of Listeria monocytogenes into the enterocytelike cell line Caco-2 [J].
Conte, MP ;
Longhi, C ;
Polidoro, M ;
Petrone, G ;
Buonfiglio, V ;
DiSanto, S ;
Papi, E ;
Seganti, L ;
Visca, P ;
Valenti, P .
INFECTION AND IMMUNITY, 1996, 64 (09) :3925-3929
[8]   Interactions of the bacterial pathogen Listeria monocytogenes with mammalian cells: Bacterial factors, cellular ligands, and signaling [J].
Cossart, P .
FOLIA MICROBIOLOGICA, 1998, 43 (03) :291-303
[9]   Utilization of iron-catecholamine complexes involving ferric reductase activity in Listeria monocytogenes [J].
Coulanges, V ;
Andre, P ;
Ziegler, O ;
Buchheit, L ;
Vidon, DJM .
INFECTION AND IMMUNITY, 1997, 65 (07) :2778-2785
[10]   Esculetin antagonizes iron-chelating agents and increases the virulence of Listeria monocytogenes [J].
Coulanges, V ;
Andre, P ;
Vidon, DJM .
RESEARCH IN MICROBIOLOGY, 1996, 147 (09) :677-685