Flow injection determination of papaverine based on its sensitizing effect on the chemiluminescence reaction of permanganate-sulfite

被引：29

作者：

Zhuang, YF ^{[1
]}

Zhang, SC ^{[1
]}

Yu, JS ^{[1
]}

Ju, HX ^{[1
]}

机构：

[1] Nanjing Univ, State Key Lab Coordinat Chem, Inst Sci Anal, Dept Chem, Nanjing 210093, Peoples R China

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2003年 / 375卷 / 02期

基金：

中国国家自然科学基金;

关键词：

chemiluminescence; papaverine; permanganate; suffite; flow injection analysis;

D O I：

10.1007/s00216-002-1651-x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel chemiluminescence (CL) method for the determination of papaverine (PAP) has been developed by combining the flow injection technique and its sensitizing effect on the weak CL reaction between sulfite and acidic perinanganate. A mechanism for the CL reaction has been proposed on the basis of fluorescent and chemiluminescence spectra. The CL response is proportional to the concentration of PAP over the range 0.2-10 mumol L-1. The detection limit of PAP is 0.10 mumol L-1 (3 s) with a relative standard deviation (RSD) of 2.0% for 10 repetitive determinations of 1.0 mumol L-1 PAP. Interferences from other alkaloids in the opium, such as morphine and codeine, are negligible except that of narcotine. The method has been satisfactorily used for the determination of PAP in injections and compound liquorice tablets.

引用

页码：281 / 286

页数：6

共 39 条

[1] DETERMINATION OF MORPHINE IN BODY-FLUIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH CHEMILUMINESCENCE DETECTION [J].