Paeoniflorin protects against concanavalin A-induced hepatitis in mice

被引:79
作者
Chen, Mingsheng [1 ]
Cao, Lijun [1 ]
Luo, Yijun [1 ]
Feng, Xiaofeng [2 ]
Sun, Lu [1 ]
Wen, Min [3 ]
Peng, Shaobin [3 ]
机构
[1] 113 Hosp Peoples Liberat Army, Dept Anesthesiol, Ningbo 315040, Zhejiang, Peoples R China
[2] 113 Hosp Peoples Liberat Army, Dept Gastroenterol, Ningbo 315040, Zhejiang, Peoples R China
[3] 113 Hosp Peoples Liberat Army, Dept Stomatol, Ningbo 315040, Zhejiang, Peoples R China
关键词
Paeoniflorin; Concanavalin A; Hepatitis; Lymphocyte; TLR4; NF-kappa B; INDUCED LIVER-INJURY; CONA-INDUCED HEPATITIS; ACUTE LUNG INJURY; NF-KAPPA-B; IMPROVES SURVIVAL; GENE-EXPRESSION; PATHWAYS; TLR4; RECEPTOR; INHIBITION;
D O I
10.1016/j.intimp.2014.11.006
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
071005 [微生物学]; 100108 [医学免疫学];
摘要
Paeoniflorin (PF) is one of the main effective components of the total glucosides of peony, which has been reported to have anti-inflammatory ability. However, the effects of paeoniflorin on concanavalin A (Con A)-induced hepatitis have not been carefully examined. The aim of this study was to investigate the protective effect of paeoniflorin and elucidate potential mechanisms of paeoniflorin on Con A-induced hepatitis. C57BL/6 mice were divided randomly into the following four experimental groups: PBS group, PF group, Con A group, and Con A + PF group. Mice received paeoniflorin (50 mg/kg) by tail vein before Con A intravenous administration. We found that paeoniflorin pretreatment can significantly reduce the elevated plasma aminotransferase levels and liver necrosis in Con A-induced hepatitis. Also, paeoniflorin pretreatment suppressed the secretion of proinflainmatory cytokines (TNF-alpha, INF-gamma, IL-6), compared with Con A group. Meanwhile, paeoniflorin pretreatment decreased CD4(+), CD8(+) and NKT cell infiltration in the liver. Besides, we observed that paeoniflorin pretreatment can decrease the expression level of Toll-like receptor CUR) 4 mRNA or protein in liver tissues. Further results showed that paeoniflorin pretreatment was capable of suppressing the activation of the NF-kappa B pathway by inhibiting I kappa B alpha kinase and p65 phosphorylation in Con A-induced liver injury. These results suggest that paeoniflorin pretreatment protects mice against Con A-induced liver injury via inhibition of several inflammatory mediators and, at least in part, by suppressing CD4(+), CD8(+) and NKT cell infiltration in liver. The beneficial effect of paeoniflorin may be related to the downregulation of TLR4 expression and the inhibition of NF-kappa B activation. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:42 / 49
页数:8
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