Effect of the natural antioxidant 2"-O-glycosylisovitexin on superoxide and hydroxyl radical generation

The inhibitory effect of 2 "-O-glycosyl isovitexin (2 "-O-GrV) isolated from green barley leaves on superoxide and hydroxyl radical generation was investigated using electron spin resonance spectroscopy. 2 "-O-GIV exhibited dose-related inhibitory activity both towards superoxide generation and hydroxyl radical generation. 2 "-O-GIV inhibited superoxide formation by 97% at a level of 25 mM and inhibited hydroxyl radical formation by 91% at a level of 500 mu M. The results suggest that the inhibitory activity of 2 "-O-GIV is due to trapping radicals. (C) 2000 Elsevier Science Ltd. All rights reserved.