Isolation and characterization of mouse-human microcell hybrid cell clones permissive for infectious HIV particle release

被引:6
作者
Coskun, Ayse K.
van Maanen, Marc
Janka, David
Stockton, David
Stankiewicsz, Pawel
Yatsenko, Svetlana
Sutton, Richard E.
机构
[1] Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA
[2] Baylor Coll Med, Dept Mol Genet, Houston, TX 77030 USA
[3] Baylor Coll Med, Dept Med, Div Infect Dis, Houston, TX 77030 USA
[4] Baylor Coll Med, Ctr Cell & Gene Therapy, Houston, TX 77030 USA
关键词
human immunodeficiency virus; murine model; capsid production; microcell hybrids; IMMUNODEFICIENCY-VIRUS TYPE-1; MURINE CELLS; MEMBRANE-BINDING; REV FUNCTION; HOST-RANGE; MATRIX; REPLICATION; PROTEIN; EXPORT; GAG;
D O I
10.1016/j.virol.2006.12.015
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Mouse cells are non-permissive to human immunodeficiency virus type I (HIV) in that there is a pronounced post-integration block to viral replication. We have recently demonstrated that mouse-human somatic cell hybrids that contain human chromosome 2 increase both HIV Capsid (CA) production and infectious virus release. Here we report on the isolation of three mouse-human microcell hybrids (MCHs) that behave similarly, starting from a pool of 500 MCH clones. Release of virus was specific to HIV and cell revertants that no longer contained any human chromosome fragments did not release CA or infectious virus. Two of the three cell clones were identical as judged by PCR STS content and fluorescence in situ hybridization (FISH) and contained a single 2-12 human chromosome chimera. The third cell clone only contained human chromosome 12, as determined by PCR, FISH, and microarray analyses. There were no consistent differences in Gag protein and spliced/ unspliced viral RNA levels between mouse cell lines. CMV promoter-driven, codon-optimized gag-pol had no effect on infectious HIV release from these mouse cells, despite allowing Gag targeting and increasing CA production. These permissive mouse-human MCHs and their corresponding non-permissive revertants may prove useful for mechanistic studies and also for identifying the responsible gene(s) or factor(s) involved in the production of HIV. (C) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:283 / 293
页数:11
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