Optimizing TiO2-based phosphopeptide enrichment for automated multidimensional liquid chromatography coupled to tandem mass spectrometry

被引：118

作者：

Cantin, Greg T.

Shock, Teresa R.

Park, Sung Kyu

Madhani, Hiten D.

Yates, John R., III

机构：

[1] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

[2] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA

来源：

ANALYTICAL CHEMISTRY | 2007年 / 79卷 / 12期

关键词：

D O I：

10.1021/ac0618730

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An automated online multidimensional liquid chromatography system coupled to ESI-based tandem mass spectrometry was used to assess the effectiveness of TiO2 in the enrichment of phosphopeptides from tryptic digests of protein mixtures. By monitoring the enrichment of phosphopeptides, an optimized set of loading, wash, and elution conditions were realized for TiO2. A comparison of TiO2 with other resins used for phosphopeptide enrichment, Fe(III)-IMAC and ZrO2, was also carried out using tryptic digests of both simple and moderately complex protein mixtures; where TiO2 was shown to be superior in performance.

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收藏

页码：4666 / 4673

页数：8

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