Agouti C57BL/6N embryonic stem cells for mouse genetic resources

被引：286

作者：

Pettitt, Stephen J. ^{[1
]}

Liang, Qi ^{[1
]}

Rairdan, Xin Y. ^{[2
]}

Moran, Jennifer L. ^{[3
]}

Prosser, Haydn M. ^{[1
]}

Beier, David R. ^{[3
]}

Lloyd, Kent C. ^{[2
]}

Bradley, Allan ^{[1
]}

Skarnes, William C. ^{[1
]}

机构：

[1] Wellcome Trust Sanger Inst, Cambridge, England

[2] Univ Calif Davis, Sch Vet Med, Ctr Comparat Med, Davis, CA 95616 USA

[3] Harvard Univ, Brigham & Womens Hosp, Sch Med, Div Genet, Boston, MA 02115 USA

来源：

NATURE METHODS | 2009年 / 6卷 / 07期

基金：

美国国家卫生研究院; 英国惠康基金;

关键词：

MICE; ESTABLISHMENT; LINE; CHROMOSOME; MUTATIONS;

D O I：

10.1038/nmeth.1342

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.

引用

收藏

页码：493 / U34

页数：4

相关论文

共 21 条

[1] Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines [J].

Auerbach, W ;

Dunmore, JH ;

Fairchild-Huntress, V ;

Fang, Q ;

Auerbach, AB ;

Huszar, D ;

Joyner, AL .

BIOTECHNIQUES, 2000, 29 (05) :1024-+

[2] MOLECULAR ANALYSIS OF REVERSE MUTATIONS FROM NONAGOUTI (A) TO BLACK-AND-TAN (A(T)) AND WHITE-BELLIED AGOUTI (A(W)) REVEALS ALTERNATIVE FORMS OF AGOUTI TRANSCRIPTS [J].

BULTMAN, SJ ;

KLEBIG, ML ;

MICHAUD, EJ ;

SWEET, HO ;

DAVISSON, MT ;

WOYCHIK, RP .

GENES & DEVELOPMENT, 1994, 8 (04) :481-490

[3] Improved generation of C57BL/6J mouse embryonic stem cells in a defined serum-free media [J].

Cheng, J ;

Dutra, A ;

Takesono, A ;

Garrett-Beal, L ;

Schwartzberg, PL .

GENESIS, 2004, 39 (02) :100-104

[4] A mouse for all reasons [J].

Collins, Francis S. .

CELL, 2007, 128 (01) :9-13

[5] Mini-λ:: a tractable system for chromosome and BAC engineering [J].

Court, DL ;

Swaminathan, S ;

Yu, DG ;

Wilson, H ;

Baker, T ;

Bubunenko, M ;

Sawitzke, J ;

Sharan, SK .

GENE, 2003, 315 :63-69

[6] MUTATIONS AT AGOUTI LOCUS IN MOUSE [J].

DICKIE, MM .

JOURNAL OF HEREDITY, 1969, 60 (01) :20-&

[7]

Farley FW, 2000, GENESIS, V28, P106, DOI 10.1002/1526-968X(200011/12)28:3/4<106::AID-GENE30>3.0.CO

[8]

2-T

[9] Large-scale gene trapping in C57BL/6N mouse embryonic stem cells [J].

Hansen, Gwenn M. ;

Markesich, Diane C. ;

Burnett, Michael B. ;

Zhu, Qichao ;

Dionne, Karen M. ;

Richter, Lizabeth J. ;

Finnell, Richard H. ;

Sands, Arthur T. ;

Zambrowicz, Brian P. ;

Abuin, Alejandro .

GENOME RESEARCH, 2008, 18 (10) :1670-1679

[10] Genetic variation in C57BL/6 ES cell lines and genetic instability in the Bruce4 C57BL/6 ES cell line [J].

Hughes, Elizabeth D. ;

Qu, Yun Yan ;

Genik, Suzanne J. ;

Lyons, Robert H. ;

Pacheco, Christopher D. ;

Lieberman, Andrew P. ;

Samuelson, Linda C. ;

Nasonkin, Igor O. ;

Camper, Sally A. ;

Van Keuren, Margaret L. ;

Saunders, Thomas L. .

MAMMALIAN GENOME, 2007, 18 (08) :549-558