Towards direct monitoring of discrete events in a catalytic cycle at the single molecule level

被引:31
作者
Ameloot, Rob [1 ]
Roeffaers, Maarten [2 ]
Baruah, Mukulesh [2 ]
De Cremer, Gert [1 ]
Sels, Bert [1 ]
De Vos, Dirk [1 ]
Hofkens, Johan [2 ]
机构
[1] Katholieke Univ Leuven, Dept Microbial & Mol Syst, Ctr Surface Chem & Catalysis, B-3001 Leuven, Belgium
[2] Katholieke Univ Leuven, Dept Chem, B-3001 Leuven, Belgium
关键词
FLUORESCENCE SPECTROSCOPY; ZSM-5; CRYSTALS; ENZYME; KINETICS;
D O I
10.1039/b821657f
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The binding state dependent quenching behaviour of functionalized dyes opens perspectives on directly monitoring binding and dissociation events in a catalytic cycle at the single molecule level.
引用
收藏
页码:453 / 456
页数:4
相关论文
共 23 条
[1]   Toward the single-molecule investigation of organometallic reaction mechanisms: Single-molecule imaging of fluorophore-tagged palladium(II) complexes [J].
Canham, Stephen M. ;
Bass, Jonathan Y. ;
Navarro, Oscar ;
Lim, Sung-Gon ;
Das, Neeladri ;
Blum, Suzanne A. .
ORGANOMETALLICS, 2008, 27 (10) :2172-2175
[2]  
Daffy LM, 1998, CHEM-EUR J, V4, P1810, DOI 10.1002/(SICI)1521-3765(19980904)4:9<1810::AID-CHEM1810>3.0.CO
[3]  
2-Y
[4]   Dynamic disorder and stepwise deactivation in a chymotrypsin catalyzed hydrolysis reaction [J].
De Cremer, Gert ;
Roeffaers, Maarten B. J. ;
Baruah, Mukulesh ;
Sliwa, Michel ;
Sels, Bert F. ;
Hofkens, Johan ;
De Vos, Dirk E. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (50) :15458-+
[5]   The fluctuating enzyme:: a single molecule approach [J].
Edman, L ;
Földes-Papp, Z ;
Wennmalm, S ;
Rigler, R .
CHEMICAL PHYSICS, 1999, 247 (01) :11-22
[6]   Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited [J].
English, BP ;
Min, W ;
van Oijen, AM ;
Lee, KT ;
Luo, GB ;
Sun, HY ;
Cherayil, BJ ;
Kou, SC ;
Xie, XS .
NATURE CHEMICAL BIOLOGY, 2006, 2 (02) :87-94
[7]  
KIEL A, 2007, P SOC PHOTO-OPT INS, P6444
[8]   Direct monitoring of formation and dissociation of individual metal complexes by single-molecule fluorescence spectroscopy [J].
Kiel, Alexander ;
Kovacs, Janos ;
Mokhir, Andriy ;
Kraemer, Roland ;
Herten, Dirk-Peter .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (18) :3363-3366
[9]   Single-molecule enzymatic dynamics [J].
Lu, HP ;
Xun, LY ;
Xie, XS .
SCIENCE, 1998, 282 (5395) :1877-1882
[10]   Exploration of single molecule events in a haloperoxidase and its biomimic:: Localization of halogenation activity [J].
Martinez, Virginia Martinez ;
De Cremer, Gert ;
Roeffaers, Maarten B. J. ;
Sliwa, Michel ;
Baruah, Mukulesh ;
De Vos, Dirk E. ;
Hofkens, Johan ;
Sels, Bert F. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (40) :13192-13193