HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF NICOTINE AND ITS URINARY METABOLITES VIA THEIR 1,3-DIETHYL-2-THIOBARBITURIC ACID-DERIVATIVES

被引：45

作者：

RUSTEMEIER, K

DEMETRIOU, D

SCHEPERS, G

VONCKEN, P

机构：

[1] INBIFO Institut für Biologische Forschung, W-5000 Köln 90

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 613卷 / 01期

关键词：

D O I：

10.1016/0378-4347(93)80201-E

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The 1,3-diethyl-2-thiobarbituric acid (DETBA) assay for nicotine metabolites has been improved so that it can be used to determine the concentrations of nicotine and up to 12 metabolites in the urine of humans and laboratory animals, including phase 2 metabolites. The products of beta-glucuronidase cleavage found in human urine were mainly trans-3'-hydroxycotinine, cotinine, and a small amount of nicotine. Following isolation, spectroscopic analyses showed the structure of the nicotine DETBA derivative to be the one-to-one ring-opening product of DEBTA and the cyanopyridinium salt of nicotine.

引用

页码：95 / 103

页数：9

共 14 条

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