AN ISOLEUCYL-TRANSFER-RNA SYNTHETASE GENE FROM CAMPYLOBACTER-JEJUNI

被引：3

作者：

HONG, YW ^{[1
]}

WONG, T ^{[1
]}

BOURKE, B ^{[1
]}

CHAN, VL ^{[1
]}

机构：

[1] UNIV TORONTO,DEPT MICROBIOL,TORONTO,ON M5S 1A8,CANADA

来源：

MICROBIOLOGY-UK | 1995年 / 141卷

关键词：

ISOLEUCYL-TRANSFER-RNA SYNTHETASE (ILERS); ALIGNMENT OF BACTERIAL ILERS; CONSERVED MOTIFS;

D O I：

10.1099/13500872-141-10-2561

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A complete isoleucyl-tRNA synthetase gene (ileS) of Campylobacter jejuni was isolated from a C. jejuni TGH9011 genomic DNA library constructed in pBluescript. The complete coding sequence, flanking regions and transcription start point were determined. The deduced isoleucyl-tRNA synthetase (IleRS) had 917 amino acids with a molecular mass of 105399 Da. which was consistent with the observed sire of 105 kDa in Escherichia coli maxicells. The ileS gene was mapped onto the physical map of the C. jejuni genome. Alignment of the C. jejuni IleRS sequence with six other bacterial IleRS sequences and two lower eukaryotic IleRS sequences identified seven conserved motifs, including the two signature sequences, HIGH and KMSKS, of class I aminoacyl-tRNA synthetases.

引用

页码：2561 / 2567

页数：7

共 46 条

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