DETERMINATION OF SULFIDOPEPTIDE LEUKOTRIENES IN BIOLOGICAL-FLUIDS BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：62

作者：

BALAZY, M ^{[1
]}

MURPHY, RC ^{[1
]}

机构：

[1] UNIV COLORADO,HLTH SCI CTR,DEPT PHARMACOL C236,4200 E 9TH AVE,DENVER,CO 80262

来源：

ANALYTICAL CHEMISTRY | 1986年 / 58卷 / 06期

关键词：

BIOLOGICAL MATERIALS - Chromatographic Analysis - SPECTROSCOPIC ANALYSIS - Applications;

D O I：

10.1021/ac00297a026

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fast, sensitive, and accurate assay for the quantitative determination of leukotrienes LTC//4, LTD//4, and LTE//4 in biological fluids by capillary gas chromatography and electron capture mass spectrometry has been developed. The assay is based upon the two-step reduction of these molecules using hydrogen and rhodium on alumina as catalyst to effect efficient saturation of the leukotriene double bonds followed by Birch reduction (lithium in methylamine) to complete thioester cleavage. The average yield of 5-hydroxyeicosanoic acid from these leukotrienes has been found to be 80 plus or minus 5% and 96 plus or minus 3% after the first and second step or reduction, respectively.

引用

页码：1098 / 1101

页数：4

共 24 条

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