DIRECT OBSERVATION OF IMMUNOGLOBULIN ADSORPTION DYNAMICS USING THE ATOMIC FORCE MICROSCOPE

被引：107

作者：

LIN, JN

DRAKE, B

LEA, AS

HANSMA, PK

ANDRADE, JD

机构：

[1] UNIV UTAH,DEPT BIOENGN,SALT LAKE CITY,UT 84112

[2] UNIV UTAH,CTR BIOPOLYMERS INTERFACES,SALT LAKE CITY,UT 84112

[3] UNIV CALIF SANTA BARBARA,DEPT PHYS,SANTA BARBARA,CA 93106

来源：

LANGMUIR | 1990年 / 6卷 / 02期

关键词：

D O I：

10.1021/la00092a036

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Atomic force microscopic images of a murine antifluorescyl monoclonal antibody (IgG 4-4-20) depositing from solution onto freshly cleaved mica were observed in real-time. These images clearly indicate a cooperative adsorption process, not a random one. Only IgG aggregates formed stable deposits, whereas isolated molecules desorbed readily from the surface. Subsequent adsorption occurred adjacent to the aggregates, forming ridges, and eventually a near monolayer was produced. Additional layers deposit only after the initial monolayer adsorption was nearly complete. Desorption of the IgG molecules in a distilled water medium was not observed. © 1990, American Chemical Society. All rights reserved.

引用

页码：509 / 511

页数：3