SYNTHETIC DNA BENDING SEQUENCES INCREASE THE RATE OF INVITRO TRANSCRIPTION INITIATION AT THE ESCHERICHIA-COLI LAC-PROMOTER

被引：136

作者：

GARTENBERG, MR

CROTHERS, DM

机构：

[1] Department of Chemistry, Yule University New Haven

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 219卷 / 02期

关键词：

DNA BENDING; A-TRACTS; TRANSCRIPTION; LAC-PROMOTER; SUPERCOILED DNA;

D O I：

10.1016/0022-2836(91)90563-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Appropriately phased DNA bending sequences replacing the CAP binding site upstream from the lac promoter increase by roughly tenfold the rate of specific transcription initiation from a superhelical promoter template in vitro; promoter occlusion results from polymerase binding to the upstream (dA)n · (dT)n tracts, but this phenomenon is not responsible for the observed phase-dependent transcriptional activity. The rates of open complex formation at both P1 and P2 promoters respond in a similar phase-dependent way to the synthetic curved DNA sequences. © 1991.

引用

页码：217 / 230

页数：14

共 51 条

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