AN INTEGRATED METRIC PHYSICAL MAP OF HUMAN-CHROMOSOME-19

被引：173

作者：

ASHWORTH, LK

BATZER, MA

BRANDRIFF, B

BRANSCOMB, E

DEJONG, P

GARCIA, E

GARNES, JA

GORDON, LA

LAMERDIN, JE

LENNON, G

MOHRENWEISER, H

OLSEN, AS

SLEZAK, T

CARRANO, AV

机构：

[1] Human Genome Center, L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA, 94551

[2] Roswell Park Cancer Institute, Buffalo, New York

来源：

NATURE GENETICS | 1995年 / 11卷 / 04期

关键词：

D O I：

10.1038/ng1295-422

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A metric physical map of human chromosome 19 has been generated. The foundation of the map is sets of overlapping cosmids (contigs) generated by automated fingerprinting spanning over 95% of the euchromatin, about 50 megabases (Mb). Distances between selected cosmid clones were estimated using fluorescence in situ hybridization in sperm pronuclei, providing both order and distance between contigs. An average inter-marker separation of 230 kb has been obtained across the non-centromeric portion of the chromosome. Various types of larger insert clones were used to span gaps between contigs. Currently, the map consists of 51 'islands' containing multiple clone types, whose size, order and relative distance are known. Over 450 genes, genetic markers, sequence tagged sites (STSs), anonymous cDNAs, and other markers have been localized. In addition, EcoRI restriction maps have been generated for >41 Mb (similar to 83%) of the chromosome.

引用

页码：422 / 427

页数：6

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