IDENTIFICATION AND CHARACTERIZATION OF 2 ISOFORMS OF AN ENDOTHELIN-CONVERTING ENZYME-1

被引：116

作者：

SHIMADA, K

TAKAHASHI, M

IKEDA, M

TANZAWA, K

机构：

[1] SANKYO CO LTD,BIOL RES LABS,SHINAGAWA KU,TOKYO 140,JAPAN

[2] SANKYO CO LTD,PHARMACOL & MOLEC BIOL RES LABS,SHINAGAWA KU,TOKYO 140,JAPAN

来源：

FEBS LETTERS | 1995年 / 371卷 / 02期

关键词：

ENDOTHELIN-CONVERTING ENZYME; ENDOTHELIN; ALTERNATIVE SPLICING; RAT ECE-1-BETA;

D O I：

10.1016/0014-5793(95)00886-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the cloning and sequencing of 5'-terminal region of a beta form of rat ECE-1 cDNA which is different only in its N-terminal amino-acid sequence to the cDNA we have cloned previously (alpha form [K, Shinada et al, (1994) J, Biol, Chem, 269, 18275-18278]). No significant difference was found in the specific activity and substrate specificity between the two isoforms. The expression level of ECE-1 alpha mRNA was higher than that of ECE-1 beta in various rat cells and tissues, suggesting that the physiologically important isoform is ECE-1 alpha, The present findings verified the presence of two forms of ECE-1 over many species, which are created probably through alternative splicing.

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页码：140 / 144

页数：5

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