A PROTEIN COMPLEX MEDIATING MESSENGER-RNA DEGRADATION IN ESCHERICHIA-COLI

被引：203

作者：

PY, B

CAUSTON, H

MUDD, EA

HIGGINS, CF

机构：

[1] UNIV OXFORD,JOHN RADCLIFFE HOSP,INST MOLEC MED,IMPERIAL CANC RES FUND LABS,OXFORD OX3 9DU,ENGLAND

[2] UNIV OXFORD,JOHN RADCLIFFE HOSP,NUFFIELD DEPT CLIN BIOCHEM,OXFORD OX3 9DU,ENGLAND

来源：

MOLECULAR MICROBIOLOGY | 1994年 / 14卷 / 04期

基金：

英国惠康基金;

关键词：

D O I：

10.1111/j.1365-2958.1994.tb01309.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

mRNA degradation in Escherichia coli is mediated by a combination of exo- and endoribonucleases. We present evidence for a multiprotein complex which includes at least two enzymes that play important roles in mRNA degradation: the exoribonuclease polynucleotide phosphorylase (PNPase) and the endoribonuclease RNase E. An activity which impedes the processive activity of PNPase at stem-loop structures also appears to be associated with the complex. This complex is estimated to have a molecular mass of about 500 kDa and includes several additional polypeptides whose functions are unknown. The identification of a complex which includes several activities associated with mRNA degradation has implications for the mechanisms and co-ordinated control of mRNA degradation.

引用

页码：717 / 729

页数：13

共 44 条

[11] POLYNUCLEOTIDE PHOSPHORYLASE AND RIBONUCLEASE-II ARE REQUIRED FOR CELL VIABILITY AND MESSENGER-RNA TURNOVER IN ESCHERICHIA-COLI K-12
DONOVAN, WP
KUSHNER, SR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (01) : 120 - 124
[12] SPECIFICITY OF ESCHERICHIA-COLI ENDORIBONUCLEASE RNASE-E - INVIVO AND INVITRO ANALYSIS OF MUTANTS IN A BACTERIOPHAGE-T4 MESSENGER-RNA PROCESSING SITE
EHRETSMANN, CP
CARPOUSIS, AJ
KRISCH, HM
[J]. GENES & DEVELOPMENT, 1992, 6 (01) : 149 - 159
[13] THE SURFACE-LOCATED YOPN PROTEIN IS INVOLVED IN CALCIUM SIGNAL TRANSDUCTION IN YERSINIA-PSEUDOTUBERCULOSIS
FORSBERG, A
VIITANEN, AM
SKURNIK, M
WOLFWATZ, H
[J]. MOLECULAR MICROBIOLOGY, 1991, 5 (04) : 977 - 986
[14] STRUCTURAL-ANALYSIS AND INVITRO PROCESSING TO P5 RIBOSOMAL-RNA OF A 9S RNA MOLECULE ISOLATED FROM AN RNE MUTANT OF ESCHERICHIA-COLI
GHORA, BK
APIRION, D
[J]. CELL, 1978, 15 (03) : 1055 - 1066
[15] PCNB IS REQUIRED FOR THE RAPID DEGRADATION OF RNAI, THE ANTISENSE RNA THAT CONTROLS THE COPY NUMBER OF COLE1-RELATED PLASMIDS
HE, L
SODERBOM, F
WAGNER, EGH
BINNIE, U
BINNS, N
MASTERS, M
[J]. MOLECULAR MICROBIOLOGY, 1993, 9 (06) : 1131 - 1142
[16] HIGGINS CF, 1993, CONTROL MESSENGER RN, P13
[17] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +
[18] THE RATE OF PROCESSING AND DEGRADATION OF ANTISENSE RNAI REGULATES THE REPLICATION OF COIE1-TYPE PLASMIDS INVIVO
LINCHAO, S
COHEN, SN
[J]. CELL, 1991, 65 (07) : 1233 - 1242
[19] RAPID MAPPING OF CONDITIONAL AND AUXOTROPHIC MUTATIONS IN ESCHERICHIA-COLI K-12
LOW, B
[J]. JOURNAL OF BACTERIOLOGY, 1973, 113 (02) : 798 - 812
[20] MESSENGER-RNA DEGRADATION BY PROCESSIVE 3'-5' EXORIBONUCLEASES INVITRO AND THE IMPLICATIONS FOR PROKARYOTIC MESSENGER-RNA DECAY INVIVO
MCLAREN, RS
NEWBURY, SF
DANCE, GSC
CAUSTON, HC
HIGGINS, CF
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1991, 221 (01) : 81 - 95

← 1 2 3 4 5 →